ArticleGene therapy2001
Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- CAR-T cell potency: from structural elements to vector backbone components.Biomarker research · 2022Review
- HOXB4 IncreasesTransfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2017Article
- The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- MGMT enrichment and second gene co-expression in hematopoietic progenitor cells using separate or dual-gene lentiviral vectors.Virus research · 2015Article
- Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications.BMC biotechnology · 2013Article
- Protein coexpression using FMDV 2A: effect of "linker" residues.BioMed research international · 2013Article
- T-cell receptor retrogenic mice: a rapid, flexible alternative to T-cell receptor transgenic mice.Immunology · 2012Review
- Dynamic HoxB4-regulatory network during embryonic stem cell differentiation to hematopoietic cells.Blood · 2012Article
- Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer.PloS one · 2011Article
- Signed outside: a surface marker system for transgenic cytoplasmic proteins.Gene therapy · 2010Article
- Successful treatment of metachromatic leukodystrophy using bone marrow transplantation of HoxB4 overexpressing cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- TCR transgenes and transgene cassettes for TCR gene therapy: status in 2008.Cancer immunology, immunotherapy : CII · 2009Review
- Overexpression of the cellular DEK protein promotes epithelial transformation in vitro and in vivo.Cancer research · 2009Article
- DEK proto-oncogene expression interferes with the normal epithelial differentiation program.The American journal of pathology · 2009Article
- Enhanced functionality of T cell receptor-redirected T cells is defined by the transgene cassette.Journal of molecular medicine (Berlin, Germany) · 2008Article
- Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells.Blood · 2008Article
- Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI.Virology journal · 2006Article
- HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters.Nature biotechnology · 2005Article
- Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector.Nature biotechnology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retroviral vector-mediated expression of the homeoboxgene, HoxB4, in hematopoietic cells has been reported to mediate a benign expansion of gene-modified hematopoietic stem and precursor cells in vivo. In the present study, we used a novel coexpression strategy for coordinated expression of HoxB4 along with a cytoplasmic protein from a retroviral vector. The novel coexpression strategy, based on cotranslational protein separation mediated by the 2A sequence of foot-and-mouth disease virus (FMDV), allows an indirect quantification of HoxB4 expression levels when inserting a reporter such as the enhanced green fluorescent protein (GFP) in the retroviral vector. Presence of the 2A sequence did not interfere with the correct subcellular localization of HoxB4 (nuclear) and GFP (cytoplasmic), nor with the titer of bicistronic vectors, and mediated functional long-term coexpression (at least 1 year) of GFP and HoxB4 after transplantation of transduced mouse bone marrow cells in mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.