ArticleGene therapy2001
Transplanted primary neonatal myoblasts can give rise to functional satellite cells as identified using the Myf5nlacZl+ mouse.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 98 citations in OpenAlex.
- Ageing is associated with diminished muscle re-growth and myogenic precursor cell expansion early after immobility-induced atrophy in human skeletal muscle.The Journal of physiology · 2013Trial
- Regulatory Landscapes of Muscle Satellite Cells: From Mechanism to Application.International journal of stem cells · 2025Review
- Dystrophin myonuclear domain restoration governs treatment efficacy in dystrophic muscle.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Satellite Cell at 60: The Foundation Years.Journal of neuromuscular diseases · 2021Review
- Obestatin Increases the Regenerative Capacity of Human Myoblasts Transplanted Intramuscularly in an Immunodeficient Mouse Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Cell Therapy in Myology: Dynamics of Muscle Precursor Cell Death after Intramuscular Administration in Non-human Primates.Molecular therapy. Methods & clinical development · 2017Article
- FOXO3 promotes quiescence in adult muscle stem cells during the process of self-renewal.Stem cell reports · 2014Article
- Gene and cell-mediated therapies for muscular dystrophy.Muscle & nerve · 2013Review
- The satellite cell in male and female, developing and adult mouse muscle: distinct stem cells for growth and regeneration.PloS one · 2012Article
- The muscle satellite cell at 50: the formative years.Skeletal muscle · 2011Article
- Efficient single muscle fiber isolation from alcohol-fixed adult muscle following β-galactosidase staining for satellite cell detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2011Article
- Heterogeneity in the muscle satellite cell population.Seminars in cell & developmental biology · 2010Review
- Intramuscular transplantation of human postnatal myoblasts generates functional donor-derived satellite cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- In vivo fluorescence imaging of muscle cell regeneration by transplanted EGFP-labeled myoblasts.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Defining the heterogeneity of skeletal muscle-derived side and main population cells isolated immediately ex vivo.Journal of cellular physiology · 2010Article
- Long-term survival of transplanted stem cells in immunocompetent mice with muscular dystrophy.The American journal of pathology · 2008Article
- Other model organisms for sarcomeric muscle diseases.Advances in experimental medicine and biology · 2008Review
- Article
- Muscle satellite cells adopt divergent fates: a mechanism for self-renewal?The Journal of cell biology · 2004Article
- Myostatin negatively regulates satellite cell activation and self-renewal.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myoblast transplantation is a potential therapeutic approach for the genetic modification of host skeletal muscle tissue. To be considered an effective, long-lived method of delivery, however, it is essential that at least a proportion of the transplanted cells also retain their proliferative potential. We sought to investigate whether transplanted neonatal myoblasts can contribute to the satellite cell compartment of adult skeletal muscle by using the Myf5nlacZ/+ mouse. The Myf5nlacZ/+ mouse has nlacZ targeted to the Myf5 locus resulting in beta-galactosidase activity in quiescent satellite cells. Following transplantation, beta-galactosidase-labelled nuclei were detected in host muscles, showing that donor cells had been incorporated. Significantly, beta-galactosidase-positive, and therefore donor-derived, satellite cells were detected. When placed in culture, beta-galactosidase marked myogenic cells emanated from the parent fibre. These observations demonstrate that cell transplantation not only results in the incorporation of donor nuclei into the host muscle syncytia, but also that the donor cells can become functional satellite cells. The Myf5nlacZ/+ mouse therefore provides a novel and specific marker for determining the contribution of transplanted cells to the satellite cell pool.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.