ArticleProceedings of the National Academy of Sciences of the United States of America2001
Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 107 citations in OpenAlex.
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- Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death.Metabolites · 2023Review
- Perturbing plasma membrane lipid: a new paradigm for tumor nanotherapeutics.Theranostics · 2023Review
- Knockdown of LMNB1 Inhibits the Proliferation of Lung Adenocarcinoma Cells by Inducing DNA Damage and Cell Senescence.Frontiers in oncology · 2022Article
- Development of Novel CD47-Specific ADCs Possessing High Potency Against Non-Small Cell Lung CancerFrontiers in oncology · 2022Article
- Quantification of Genetically Encoded Lipid Biosensors.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Identification of unusual phospholipids from bovine heart mitochondria by HPLC-MS/MS.Journal of lipid research · 2020Article
- Comprehensive analysis of phospholipids in the brain, heart, kidney, and liver: brain phospholipids are least enriched with polyunsaturated fatty acids.Molecular and cellular biochemistry · 2018Article
- Fatty-acyl chain profiles of cellular phosphoinositides.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Advances in Lipidomics for Cancer Biomarkers Discovery.International journal of molecular sciences · 2016Review
- Fourier Transform Mass Spectrometry and Nuclear Magnetic Resonance Analysis for the Rapid and Accurate Characterization of Hexacosanoylceramide.International journal of molecular sciences · 2016Article
- Unraveling the Composition and Behavior of Heterogeneous Lipid Nanodiscs by Mass Spectrometry.Analytical chemistry · 2016Article
- Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry.Journal of lipid research · 2016Article
- Applications of mass spectrometry for cellular lipid analysis.Molecular bioSystems · 2015Review
- A mass spectrometric study for comparative analysis and evaluation of metabolite recovery from plasma by various solvent systems.Journal of biomolecular techniques : JBT · 2012Article
- cPLA2α and EHD1 interact and regulate the vesiculation of cholesterol-rich, GPI-anchored, protein-containing endosomes.Molecular biology of the cell · 2012Article
- Lipidomics: when apocrypha becomes canonical.Current opinion in chemical biology · 2012Review
- A mouse macrophage lipidome.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Biological membranes contain an extraordinary diversity of lipids. Phospholipids function as major structural elements of cellular membranes, and analysis of changes in the highly heterogeneous mixtures of lipids found in eukaryotic cells is central to understanding the complex functions in which lipids participate. Phospholipase-catalyzed hydrolysis of phospholipids often follows cell surface receptor activation. Recently, we demonstrated that granule fusion is initiated by addition of exogenous, nonmammalian phospholipases to permeabilized mast cells. To pursue this finding, we use positive and negative mode Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) to measure changes in the glycerophospholipid composition of total lipid extracts of intact and permeabilized RBL-2H3 (mucosal mast cell line) cells. The low energy of the electrospray ionization results in efficient production of molecular ions of phospholipids uncomplicated by further fragmentation, and changes were observed that eluded conventional detection methods. From these analyses we have spectrally resolved more than 130 glycerophospholipids and determined changes initiated by introduction of exogenous phospholipase C, phospholipase D, or phospholipase A2. These exogenous phospholipases have a preference for phosphatidylcholine with long polyunsaturated alkyl chains as substrates and, when added to permeabilized mast cells, produce multiple species of mono- and polyunsaturated diacylglycerols, phosphatidic acids, and lysophosphatidylcholines, respectively. The patterns of changes of these lipids provide an extraordinarily rich source of data for evaluating the effects of specific lipid species generated during cellular processes, such as exocytosis.
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