ArticleProceedings of the National Academy of Sciences of the United States of America2001
Sorting and directed transport of membrane proteins during development of hippocampal neurons in culture.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Differentiated SH-SY5Y cells exhibit neuronal features but lack synaptic maturity.Cell death discovery · 2026Article
- Kinesin Regulation in the Proximal Axon is Essential for Dendrite-selective Transport.Molecular biology of the cell · 2024Article
- Polarized transport requires AP-1-mediated recruitment of KIF13A and KIF13B at the trans-Golgi.Molecular biology of the cell · 2024Article
- Article
- Propofol attenuates kinesin-mediated axonal vesicle transport and fusion.Molecular biology of the cell · 2022Article
- NgCAM and VAMP2 reveal that direct delivery and dendritic degradation maintain axonal polarity.Molecular biology of the cell · 2022Article
- Imaging Intracellular Trafficking in Neurons of C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population.Traffic (Copenhagen, Denmark) · 2020Article
- Spatial control of membrane traffic in neuronal dendrites.Molecular and cellular neurosciences · 2020Review
- A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport.Traffic (Copenhagen, Denmark) · 2019Article
- Structure and Function of an Actin-Based Filter in the Proximal Axon.Cell reports · 2017Article
- Adaptor Protein 2 (AP-2) complex is essential for functional axogenesis in hippocampal neurons.Scientific reports · 2017Article
- Axonal Membranes and Their Domains: Assembly and Function of the Axon Initial Segment and Node of Ranvier.Frontiers in cellular neuroscience · 2017Review
- The cellular mechanisms that maintain neuronal polarity.Nature reviews. Neuroscience · 2016Review
- Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Stochastic Subcellular Organization of Dense-Core Vesicles Revealed by Point Pattern Analysis.Biophysical journal · 2016Article
- A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations.The Journal of cell biology · 2015Article
- Barriers to the free diffusion of proteins and lipids in the plasma membrane.The Journal of cell biology · 2015Review
- Live-cell imaging of post-golgi transport vesicles in cultured hippocampal neurons.Cellular and molecular neurobiology · 2015Article
- Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.Journal of neuroscience research · 2014Article
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7 authors.
Funding
Abstract
Hippocampal neurons in culture develop morphological polarity in a sequential pattern; axons form before dendrites. Molecular differences, particularly those of membrane proteins, underlie the functional polarity of these domains, yet little is known about the temporal relationship between membrane protein polarization and morphological polarization. We took advantage of viral expression systems to determine when during development the polarization of membrane proteins arises. All markers were unpolarized in neurons before axonogenesis. In neurons with a morphologically distinguishable axon, even on the first day in culture, both axonal and dendritic proteins were polarized. The degree of polarization at these early stages was somewhat less than in mature cells and varied from cell to cell. The cellular mechanism responsible for the polarization of the dendritic marker protein transferrin receptor (TfR) in mature cells centers on directed transport to the dendritic domain. To examine the relationship between cell surface polarization and transport, we assessed the selectivity of transport by live cell imaging. TfR-green fluorescent protein-containing vesicles were already preferentially transported into dendrites at 2 days, the earliest time point we could measure. The selectivity of transport also varied somewhat among cells, and the amount of TfR-green fluorescent protein fluorescence on intracellular structures within the axon correlated with the amount of cell surface expression. This observation implies that selective microtubule-based transport is the primary mechanism that underlies the polarization of TfR on the cell surface. By 5 days in culture, the extent of polarization on the cell surface and the selectivity of transport reached mature levels.
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