ArticleProceedings of the National Academy of Sciences of the United States of America2001
The physiological significance of beta -actin mRNA localization in determining cell polarity and directional motility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers, 1 of them a synthesis that pooled it.
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Who cites it
118 citing papers in PubMed, 1 synthesis or guideline pooled it, 216 citations in OpenAlex.
- Cross-expression meta-analysis of mouse brain slices reveals coordinated gene expression across spatially adjacent cells.Genome biology · 2025Pooled it
- Subcellular localization as a driver of protein function.Nature reviews. Molecular cell biology · 2026Review
- The choreography of development: RNA localization across time and space.Development (Cambridge, England) · 2026Review
- Mechanics of blunting of actin-myosin interaction dynamics by the actinopathy-causing mutation E334Q in cytoskeletal γ-actin.The Journal of physiology · 2026Article
- The differential subcellular localization of soybean transcripts, an additional regulatory mechanism of gene activity.Genome biology · 2025Article
- Cytoplasmic localization of the mRNA encoding actin regulator, Serendipity-bioRxiv : the preprint server for biology · 2025Article
- Cross-expression meta-analysis of 695 brain samples reveals coordinated gene expression across spatially adjacent cells.bioRxiv : the preprint server for biology · 2025Article
- An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.Journal of cell science · 2024Article
- MARCKS and PI(4,5)PMolecular biology of the cell · 2024Article
- RNA trafficking and subcellular localization-a review of mechanisms, experimental and predictive methodologies.Briefings in bioinformatics · 2023Review
- The importance of mRNA (New York, N.Y.) · 2023Article
- RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein.Journal of cell science · 2023Article
- Dynamics of axonal β-actin mRNA in live hippocampal neurons.Traffic (Copenhagen, Denmark) · 2022Article
- Review
- Tagged actin mRNA dysregulation in IGF2BP1[Formula: see text] mice.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration.iScience · 2022Article
- Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells.Regenerative biomaterials · 2022Article
- Crystallin gene expression: Insights from studies of transcriptional bursting.Experimental eye research · 2021Review
- Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton.Frontiers in cell and developmental biology · 2021Review
58 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
beta-actin mRNA is localized near the leading edge in several cell types, where actin polymerization is actively promoting forward protrusion. The localization of the beta-actin mRNA near the leading edge is facilitated by a short sequence in the 3' untranslated region, the "zip code." Localization of the mRNA at this region is important physiologically. Treatment of chicken embryo fibroblasts with antisense oligonucleotides complementary to the localization sequence (zip code) in the 3' untranslated region leads to delocalization of beta-actin mRNA, alteration of cell phenotype, and a decrease in cell motility. To determine the components of this process responsible for the change in cell behavior after beta-actin mRNA delocalization, the Dynamic Image Analysis System was used to quantify movement of cells in the presence of sense and antisense oligonucleotides to the zip code. It was found that net path length and average speed of antisense-treated cells were significantly lower than in sense-treated cells. Total path length and the velocity of protrusion of antisense-treated cells were not affected compared with those of control cells. These results suggest that a decrease in persistence of direction of movement and not in velocity results from treatment of cells with zip code-directed antisense oligonucleotides. To test this, direct analysis of directionality was performed on antisense-treated cells and showed a decrease in directionality (net path/total path) and persistence of movement. Less directional movement of antisense-treated cells correlated with a unpolarized and discontinuous distribution of free barbed ends of actin filaments and of beta-actin protein. These results indicate that delocalization of beta-actin mRNA results in delocalization of nucleation sites and beta-actin protein from the leading edge followed by loss of cell polarity and directional movement.
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