ArticleProceedings of the National Academy of Sciences of the United States of America2001
Related homing endonucleases I-BmoI and I-TevI use different strategies to cleave homologous recognition sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 52 citations in OpenAlex.
- Modular determinants of cleavage preference in GIY-YIG nucleases revealed by block-based DNA shuffling and directed evolution.Nucleic acids research · 2026Article
- Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases.Human gene therapy · 2025Article
- Functional dissection of the conserved C. elegans LEM-3/ANKLE1 nuclease reveals a crucial requirement for the LEM-like and GIY-YIG domains for DNA bridge processing.Nucleic acids research · 2025Article
- Perpetuating the homing endonuclease life cycle: identification of mutations that modulate and change I-TevI cleavage preference.Nucleic acids research · 2016Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- The monomeric GIY-YIG homing endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially nick DNA.Nucleic acids research · 2013Article
- Monomeric site-specific nucleases for genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.Nucleic acids research · 2011Article
- Native homing endonucleases can target conserved genes in humans and in animal models.Nucleic acids research · 2011Article
- Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoI.PloS one · 2011Article
- Estimating the evidence of selection and the reliability of inference in unigenic evolution.Algorithms for molecular biology : AMB · 2010Article
- A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI.Nucleic acids research · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Bacteriophage T4 endonuclease II, a promiscuous GIY-YIG nuclease, binds as a tetramer to two DNA substrates.Nucleic acids research · 2009Article
- Biochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifs.Nucleic acids research · 2009Article
- A likely pathway for formation of mobile group I introns.Current biology : CB · 2009Article
- Revised nomenclature for transposable genetic elements.Plasmid · 2008Review
- Role of the interdomain linker in distance determination for remote cleavage by homing endonuclease I-TevI.Journal of molecular biology · 2008Article
- Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family.Nucleic acids research · 2008Article
- Coevolution of a homing endonuclease and its host target sequence.Journal of molecular biology · 2007Article
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2 authors at 1 institution in 1 country.
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Abstract
A typical homing endonuclease initiates mobility of its group I intron by recognizing DNA both upstream and downstream of the intron insertion site of intronless alleles, preventing the endonuclease from binding and cleaving its own intron-containing allele. Here, we describe a GIY-YIG family homing endonuclease, I-BmoI, that possesses an unusual recognition sequence, encompassing 1 base pair upstream but 38 base pairs downstream of the intron insertion site. I-BmoI binds intron-containing and intronless substrates with equal affinity but can nevertheless discriminate between the two for cleavage. I-BmoI is encoded by a group I intron that interrupts the thymidylate synthase (TS) gene (thyA) of Bacillus mojavensis s87-18. This intron resembles one inserted 21 nucleotides further downstream in a homologous TS gene (td) of Escherichia coli phage T4. I-TevI, the T4 td intron-encoded GIY-YIG endonuclease, is very similar to I-BmoI, but each endonuclease gene is inserted within a different position of its respective intron. Remarkably, I-TevI and I-BmoI bind a homologous stretch of TS-encoding DNA and cleave their intronless substrates in very similar positions. Our results suggest that each endonuclease has independently evolved the ability to distinguish intron-containing from intronless alleles while maintaining the same conserved recognition sequence centered on DNA-encoding active site residues of TS.
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