ArticleMolecular and cellular biology2001
Site-specific recognition of a 70-base-pair element containing d(GA)(n) repeats mediates bithoraxoid polycomb group response element-dependent silencing.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 94 citations in OpenAlex.
- Polycomb Group Proteins inBiology · 2026Review
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Crol contributes to PRE-mediated repression and Polycomb group proteins recruitment in Drosophila.Nucleic acids research · 2023Article
- GAGA-associated factor fosters loop formation in the Drosophila genome.Molecular cell · 2023Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- Engaging chromatin: PRC2 structure meets function.British journal of cancer · 2020Review
- Genome-wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER inPlant direct · 2019Article
- PTE, a novel module to target Polycomb Repressive Complex 1 to the human cyclin D2 (The Journal of biological chemistry · 2018Article
- Vertebrate GAF/ThPOK: emerging functions in chromatin architecture and transcriptional regulation.Cellular and molecular life sciences : CMLS · 2018Review
- A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don.Scientific reports · 2016Article
- Review
- Transcriptional read-through is not sufficient to induce an epigenetic switch in the silencing activity of Polycomb response elements.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A new world of Polycombs: unexpected partnerships and emerging functions.Nature reviews. Genetics · 2013Review
- Polycomb silencing of the Drosophila 4E-BP gene regulates imaginal disc cell growth.Developmental biology · 2013Article
- Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome.PloS one · 2013Article
- MK3 controls Polycomb target gene expression via negative feedback on ERK.Epigenetics & chromatin · 2012Article
- Genome-wide polycomb target gene prediction in Drosophila melanogaster.Nucleic acids research · 2012Article
- Quantitative analysis of polycomb response elements (PREs) at identical genomic locations distinguishes contributions of PRE sequence and genomic environment.Epigenetics & chromatin · 2011Article
- Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Polycomb group proteins act through Polycomb group response elements (PREs) to maintain silencing at homeotic loci. The minimal 1.5-kb bithoraxoid (bxd) PRE contains a region required for pairing-sensitive repression and flanking regions required for maintenance of embryonic silencing. Little is known about the identity of specific sequences necessary for function of the flanking regions. Using gel mobility shift analysis, we identify DNA binding activities that interact specifically with a multipartite 70-bp fragment (MHS-70) downstream of the pairing-sensitive sequence. Deletion of MHS-70 in the context of a 5.1-kb bxd Polycomb group response element derepresses maintenance of silencing in embryos. A partially purified binding activity requires multiple, nonoverlapping d(GA)(3) repeats for MHS-70 binding in vitro. Mutation of d(GA)(3) repeats within MHS-70 in the context of the 5.1-kb bxd PRE destabilizes maintenance of silencing in a subset of cells in vivo but gives weaker derepression than deletion of MHS-70. These results suggest that d(GA)(3) repeats are important for silencing but that other sequences within MHS-70 also contribute to silencing. Antibody supershift assays and Western analyses show that distinct isoforms of Polyhomeotic and two proteins that recognize d(GA)(3) repeats, the TRL/GAGA factor and Pipsqueak (Psq), are present in the MHS-70 binding activity. Mutations in Trl and psq enhance homeotic phenotypes of ph, indicating that TRL/GAGA factor and Psq are enhancers of Polycomb which have sequence-specific DNA binding activity. These studies demonstrate that site-specific recognition of the bxd PRE by d(GA)(n) repeat binding activities mediates PcG-dependent silencing.
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