ArticleMolecular and cellular biology2001
Characterization of the Crithidia fasciculata mRNA cycling sequence binding proteins.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A post-transcriptional respiratome regulon in trypanosomes.Nucleic acids research · 2019Article
- Ubiquitination-mediated interaction among domains is responsible for inhibition of RNA endonuclease activity of mRNA cycling sequence binding protein from L. donovani (LdCSBP).Parasitology research · 2014Article
- The mRNAs associated to a zinc finger protein from Trypanosoma cruzi shift during stress conditions.RNA biology · 2014Article
- Bloodstream form pre-adaptation to the tsetse fly in Trypanosoma brucei.Frontiers in cellular and infection microbiology · 2013Review
- The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei.Nucleic acids research · 2012Article
- Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- Genome-wide in silico screen for CCCH-type zinc finger proteins of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major.BMC genomics · 2010Article
- A mitochondrial DNA primase is essential for cell growth and kinetoplast DNA replication in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Identification of new kinetoplast DNA replication proteins in trypanosomatids based on predicted S-phase expression and mitochondrial targeting.Eukaryotic cell · 2007Article
- Complete cap 4 formation is not required for viability in Trypanosoma brucei.Eukaryotic cell · 2006Article
- Cell cycle-dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata.Eukaryotic cell · 2006Article
- Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.Nucleic acids research · 2005Article
- Presence of a poly(A) binding protein and two proteins with cell cycle-dependent phosphorylation in Crithidia fasciculata mRNA cycling sequence binding protein II.Eukaryotic cell · 2004Article
- Mitochondrial DNA ligase in Crithidia fasciculata.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Sequence elements in both the intergenic space and the 3' untranslated region of the Crithidia fasciculata KAP3 gene are required for cell cycle regulation of KAP3 mRNA.Eukaryotic cell · 2003Article
- Life without transcriptional control? From fly to man and back again.The EMBO journal · 2002Review
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Authors and funding
4 authors.
Funding
Abstract
The Crithidia fasciculata cycling sequence binding protein (CSBP) binds with high specificity to sequence elements in several mRNAs that accumulate periodically during the cell cycle. Mutations in these sequence elements abolish both cycling of the mRNA and binding of CSBP. Two genes, CSBPA and CSBPB, encoding putative subunits of CSBP have been cloned and were found to be present in tandem on the same DNA molecule and to be closely related. CSBPA and CSBPB are predicted to encode proteins with sizes of 35.6 and 42.0 kDa, respectively. Both CSBPA and CSBPB proteins have a predicted coiled-coil domain near the N terminus and a novel histidine and cysteine motif near the C terminus. The latter motif is conserved in other trypanosomatid species. Gel sieving chromatography and glycerol gradient sedimentation results indicate that CSBP has a molecular mass in excess of 200 kDa and an extended structure. Recombinant CSBPA and CSBPB also bind specifically to the cycling sequence and together can be reconstituted to give an RNA gel shift similar to that of purified CSBP. Proteins in cell extracts bind to an RNA probe containing six copies of the cycling sequence. The RNA-protein complexes contain both CSBPA and CSBPB, and the binding activity cycles in near synchrony with target mRNA levels. CSBPA and CSBPB mRNA and protein levels show little variation throughout the cell cycle, suggesting that additional factors are involved in the cyclic binding to the cycling sequence elements.
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