ArticleMolecular and cellular biology2001
Phosphorylation of MafA is essential for its transcriptional and biological properties.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 91 citations in OpenAlex.
- Review
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- Multicentric Carpotarsal Osteolysis: a Contemporary Perspective on the Unique Skeletal Phenotype.Current osteoporosis reports · 2023Review
- Mafa-dependent GABAergic activity promotes mouse neonatal apneas.Nature communications · 2022Article
- Second MAFA Variant Causing a Phosphorylation Defect in the Transactivation Domain and Familial Insulinomatosis.Cancers · 2022Article
- MafA Regulation in β-Cells: From Transcriptional to Post-Translational Mechanisms.Biomolecules · 2022Review
- Small molecule ERK5 kinase inhibitors paradoxically activate ERK5 signalling: be careful what you wish for….Biochemical Society transactions · 2020Review
- Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors.Investigative ophthalmology & visual science · 2019Article
- Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina.Scientific reports · 2019Article
- Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.Neural development · 2018Article
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- CMIP is oncogenic in human gastric cancer cells.Molecular medicine reports · 2017Article
- A Comprehensive Survey of the Roles of Highly Disordered Proteins in Type 2 Diabetes.International journal of molecular sciences · 2017Article
- Deficiency of programmed cell death 4 results in increased IL-10 expression by macrophages and thereby attenuates atherosclerosis in hyperlipidemic mice.Cellular & molecular immunology · 2016Article
- Proper activation of MafA is required for optimal differentiation and maturation of pancreatic β-cells.Best practice & research. Clinical endocrinology & metabolism · 2015Review
- Inhibition of human insulin gene transcription and MafA transcriptional activity by the dual leucine zipper kinase.Cellular signalling · 2014Article
- Cytoprotection "gone astray": Nrf2 and its role in cancer.OncoTargets and therapy · 2014Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously described the identification of quail MafA, a novel transcription factor of the Maf bZIP (basic region leucine zipper) family, expressed in the differentiating neuroretina (NR). In the present study, we provide the first evidence that MafA is phosphorylated and that its biological properties strongly rely upon phosphorylation of serines 14 and 65, two residues located in the transcriptional activating domain within a consensus for phosphorylation by mitogen-activated protein kinases and which are conserved among Maf proteins. These residues are phosphorylated by ERK2 but not by p38, JNK, and ERK5 in vitro. However, the contribution of the MEK/ERK pathway to MafA phosphorylation in vivo appears to be moderate, implicating another kinase. The integrity of serine 14 and serine 65 residues is required for transcriptional activity, since their mutation into alanine severely impairs MafA capacity to activate transcription. Furthermore, we show that the MafA S14A/S65A mutant displays reduced capacity to induce expression of QR1, an NR-specific target of Maf proteins. Likewise, the integrity of serines 14 and 65 is essential for the MafA ability to stimulate expression of crystallin genes in NR cells and to induce NR-to-lens transdifferentiation. Thus, the MafA capacity to induce differentiation programs is dependent on its phosphorylation.
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