ArticleEMBO reports2001
An artificial transcription activator mimics the genome-wide properties of the yeast Pdr1 transcription factor.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Unveiling the transcriptional control of pleiotropic drug resistance in Saccharomyces cerevisiae: Contributions of André Goffeau and his group.Yeast (Chichester, England) · 2019Review
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Reduced Glucose Sensation Can Increase the Fitness of Saccharomyces cerevisiae Lacking Mitochondrial DNA.PloS one · 2016Article
- Examining the virulence of Candida albicans transcription factor mutants using Galleria mellonella and mouse infection models.Frontiers in microbiology · 2015Article
- Multidrug resistance in fungi: regulation of transporter-encoding gene expression.Frontiers in physiology · 2014Review
- Mapping yeast transcriptional networks.Genetics · 2013Review
- Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription.Journal of molecular biology · 2013Article
- Loss of mitochondrial functions associated with azole resistance in Candida glabrata results in enhanced virulence in mice.Antimicrobial agents and chemotherapy · 2011Article
- Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata.PloS one · 2011Article
- Article
- Structure and properties of transcriptional networks driving selenite stress response in yeasts.BMC genomics · 2008Article
- Responses of pathogenic and nonpathogenic yeast species to steroids reveal the functioning and evolution of multidrug resistance transcriptional networks.Eukaryotic cell · 2008Article
- Membrane-active compounds activate the transcription factors Pdr1 and Pdr3 connecting pleiotropic drug resistance and membrane lipid homeostasis in saccharomyces cerevisiae.Molecular biology of the cell · 2007Article
- Identifying transcription factor functions and targets by phenotypic activation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis.The Plant cell · 2005Article
- Early expression of yeast genes affected by chemical stress.Molecular and cellular biology · 2005Article
- An evolutionary and functional assessment of regulatory network motifs.Genome biology · 2005Article
- Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae.Eukaryotic cell · 2004Article
- Pdr3 is required for DNA damage induction of MAG1 and DDI1 via a bi-directional promoter element.Nucleic acids research · 2004Article
- New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analysed the genome-wide regulatory properties of an artificial transcription activator in which the DNA-binding domain of the yeast transcription factor, Pdr1, was fused to the activation domain of Gal4 (Pdr1*GAD). This Pdr1*GAD chimera was put under the control of the inducible GAL1 promoter. DNA microarray analyses showed that all the target genes upregulated by the well-studied native gain-of-function Pdr1-3 mutant were similarly activated by the chimerical factor Pdr1*GAD upon galactose induction. Additionally, this kinetic approach led us not only to confirm previously published targets, but also to define a hierarchy among members of the Pdr1 regulon. Our observations prove, for the first time at the complete genome level, that the DNA-binding domain of Pdr1 is sufficient to guide its specificity. We propose that this approach could be useful for the study of new transcription factors identified in silico from sequenced organisms. Complete data are available at www.biologie.ens.fr/yeast-publi.html.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.