ArticleBMC cell biology2001
Cytosolic acidification as a signal mediating hyperosmotic stress responses in Dictyostelium discoideum.
Article in BMC cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Agglomeration: when folded proteins clump together.Biophysical reviews · 2023Review
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- Formation of Cytoplasmic Actin-Cofilin Rods is Triggered by Metabolic Stress and Changes in Cellular pH.Frontiers in cell and developmental biology · 2021Article
- Endocytosis in the adaptation to cellular stress.Cell stress · 2020Review
- The physiological regulation of macropinocytosis duringJournal of cell science · 2018Article
- A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy.eLife · 2016Article
- Article
- The arrestin-domain containing protein AdcA is a response element to stress.Cell communication and signaling : CCS · 2013Article
- Structural, catalytic and stabilizing consequences of aromatic cluster variants in human carbonic anhydrase II.Archives of biochemistry and biophysics · 2013Article
- Endocytic response of type I alveolar epithelial cells to hypertonic stress.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Tyrosine phosphorylation-mediated signaling pathways in dictyostelium.Journal of signal transduction · 2011Article
- Visualizing myosin-actin interaction with a genetically-encoded fluorescent strain sensor.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Article
- Hisactophilin is involved in osmoprotection in Dictyostelium.BMC biochemistry · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDictyostelium cells exhibit an unusual response to hyperosmolarity that is distinct from the response in other organisms investigated: instead of accumulating compatible osmolytes as it has been described for a wide range of organisms, Dictyostelium cells rearrange their cytoskeleton and thereby build up a rigid network which is believed to constitute the major osmoprotective mechanism in this organism. To gain more insight into the osmoregulation of this amoeba, we investigated physiological processes affected under hyperosmotic conditions in Dictyostelium.
resultsWe determined pH changes in response to hyperosmotic stress using FACS or 31P-NMR. Hyperosmolarity was found to acidify the cytosol from pH 7.5 to 6.8 within 5 minutes, whereas the pH of the endo-lysosomal compartment remained constant. Fluid-phase endocytosis was identified as a possible target of cytosolic acidification, as the inhibition of endocytosis observed under hypertonic conditions can be fully attributed to cytosolic acidification. In addition, a deceleration of vesicle mobility and a decrease in the NTP pool was observed.
conclusionTogether, these results indicate that hyperosmotic stress triggers pleiotropic effects, which are partially mediated by a pH signal and which all contribute to the downregulation of cellular activity. The comparison of our results with the effect of hyperosmolarity and intracellular acidification on receptor-mediated endocytosis in mammalian cells reveals striking similarities, suggesting the hypothesis of the same mechanism of inhibition by low internal pH.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.