ArticleThe Biochemical journal2001
Transforming growth factor beta-1 enhances Smad transcriptional activity through activation of p8 gene expression.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Attenuation of epicardial activation and myofibroblast abundance via the Fbln2-Nupr1b axis stimulates cardiac regeneration in zebrafish.Nature cardiovascular research · 2026Article
- Suppression of NUPR1 in fibroblast-like synoviocytes reduces synovial fibrosis via the Smad3 pathway.Journal of translational medicine · 2024Article
- Review
- Induction of NUPR1 and AP‑1 contributes to the carcinogenic potential of nickel.Oncology reports · 2021Article
- Nuclear protein 1 imparts oncogenic potential and chemotherapeutic resistance in cancer.Cancer letters · 2020Review
- The E2F transcription factors regulate tumor development and metastasis in a mouse model of metastatic breast cancer.Molecular and cellular biology · 2014Article
- Expression and roles of As-NUPR1 protein from Artemia sinica during embryo development and in response to salinity stress.Molecular biology reports · 2014Article
- Homotypic cell cannibalism, a cell-death process regulated by the nuclear protein 1, opposes to metastasis in pancreatic cancer.EMBO molecular medicine · 2012Article
- Nuclear protein 1 promotes pancreatic cancer development and protects cells from stress by inhibiting apoptosis.The Journal of clinical investigation · 2012Article
- Expression and roles of NUPR1 in cholangiocarcinoma cells.Anatomy & cell biology · 2012Article
- Stress-inducible protein p8 is involved in several physiological and pathological processes.The Journal of biological chemistry · 2010Review
- The small chromatin-binding protein p8 coordinates the association of anti-proliferative and pro-myogenic proteins at the myogenin promoter.Journal of cell science · 2009Article
- Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model.The Journal of endocrinology · 2007Article
- Developmentally regulated synthesis of p8, a stress-associated transcription cofactor, in diapause-destined embryos of Artemia franciscana.Cell stress & chaperones · 2007Article
- Recent insights into the cellular mechanisms of acute pancreatitis.Canadian journal of gastroenterology = Journal canadien de gastroenterologie · 2007Review
- Transcriptional response of a common permissive cell type to infection by two diverse alphaherpesviruses.Journal of virology · 2004Article
- Gonadotropin releasing hormone analogue (GnRHa) alters the expression and activation of Smad in human endometrial epithelial and stromal cells.Reproductive biology and endocrinology : RB&E · 2003Article
- p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth.Molecular cancer · 2003Article
- p8 is critical for tumour development induced by rasV12 mutated protein and E1A oncogene.EMBO reports · 2002Article
- Expression of the stress-associated protein p8 is a requisite for tumor development.International journal of gastrointestinal cancer · 2002Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report that exposure of mouse embryonic fibroblasts to transforming growth factor beta-1 (TGFbeta-1) (5 ng/ml) results in a strong activation of p8 mRNA expression that precedes the induction of cell growth. Involvement of the p8 promoter in the regulation was demonstrated by using a p8-chloramphenicol acetyltransferase construct. We therefore speculated that p8 might be a mediator of TGFbeta-1 in these cells. The incorporation of [(3)H]thymidine on treatment with TGFbeta-1 was indeed significantly higher in p8(+/+) fibroblasts than in p8(-/-) fibroblasts. Smad transcriptional activity was used as marker of the TGFbeta-1 signalling pathway, to probe the lower p8(-/-) response to TGFbeta-1. Two Smad-binding elements (SBEs)-luciferase constructs were transfected into p8(-/-) and p8(+/+) embryonic fibroblasts before treatment with TGFbeta-1. A lower level of Smad transactivation was observed in p8(-/-) embryonic fibroblasts, under basal conditions and after stimulation with TGFbeta-1. To test whether Smad underexpression in p8(-/-) cells was actually due to p8 depletion, p8(-/-) embryonic fibroblasts were transfected with a human p8 expression plasmid together with an SBE-luciferase construct. The expression of p8 restored Smad transactivation in unstimulated and TGFbeta-1-treated cells to the level found in p8(+/+) cells. We concluded that TGFbeta-1 activates p8 expression, which in turn enhances the Smad-transactivating function responsible for TGFbeta-1 activity.
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