ArticleThe Biochemical journal2001
Hsp70 regulates the interaction between the peroxisome targeting signal type 1 (PTS1)-receptor Pex5p and PTS1.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 57 citations in OpenAlex.
- Role of Hsp70 in Post-Translational Protein Targeting: Tail-Anchored Membrane Proteins and Beyond.International journal of molecular sciences · 2023Review
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
- The chaperone-binding activity of the mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress.Cell reports · 2021Article
- Guiding tail-anchored membrane proteins to the endoplasmic reticulum in a chaperone cascade.The Journal of biological chemistry · 2019Review
- Substrate relay in an Hsp70-cochaperone cascade safeguards tail-anchored membrane protein targeting.The EMBO journal · 2018Article
- Allostery between two binding sites in the ion channel subunit TRIP8b confers binding specificity to HCN channels.The Journal of biological chemistry · 2017Article
- Localization of Protein Kinase NDR2 to Peroxisomes and Its Role in Ciliogenesis.The Journal of biological chemistry · 2017Article
- Peroxisome protein import: a complex journey.Biochemical Society transactions · 2016Review
- Covalent Label Transfer between Peroxisomal Importomer Components Reveals Export-driven Import Interactions.The Journal of biological chemistry · 2016Article
- PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.FEBS letters · 2014Article
- Redox-regulated cargo binding and release by the peroxisomal targeting signal receptor, Pex5.The Journal of biological chemistry · 2013Article
- Peroxisome degradation in mammals: mechanisms of action, recent advances, and perspectives.Frontiers in physiology · 2013Article
- Peroxisomes and aging.Biochimica et biophysica acta · 2006Review
- Extreme secretion: protein translocation across the archael plasma membrane.Journal of bioenergetics and biomembranes · 2004Review
- A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Peroxisome senescence in human fibroblasts.Molecular biology of the cell · 2002Article
- Opinion: peroxisomal-protein import: is it really that complex?Nature reviews. Molecular cell biology · 2002Review
- Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The peroxisome targeting signal type 1 (PTS1) receptor, Pex5p, of the tetratricopeptide repeat (TPR) motif family is located mostly in the cytosol and mediates the translocation of PTS1 proteins to peroxisomes. As a step towards understanding the mechanisms of protein import into peroxisomes, we investigated the molecular mechanisms involved in PTS1 recognition by Pex5p with regard to requirement of energy and cytosolic factors, using cell-free synthesized acyl-CoA oxidase (AOx) as a PTS1 cargo protein, together with Pex5p and heat-shock protein (Hsp)70 from rat liver. Pex5p was partly associated with peroxisomes of rat liver, was resistant to washing with a high concentration of salt and to alkaline extraction and was inaccessible to protease added externally. Pex5p bound to AOx in an ATP-dependent manner. AOx synthesized in a cell-free translating system from rabbit reticulocyte lysate was imported into peroxisomes without being supplemented with Pex5p and Hsp70, implying that peroxisome-associated Pex5p was released from the membranes and functional in this in vitro import assay. Antibodies against Pex5p and Hsp70 inhibited AOx import. In contrast, AOx synthesized in a wheat-germ lysate required the external addition of Pex5p for import, in which Hsp70 augmented the AOx import. The TPR domain of Pex5p was revealed to bind to the N-terminal part in an Hsp70-independent manner, whereas mutual interaction of the TPR region was noted in the presence of Hsp70. Hsp70 interacted with the TPR domain of Pex5p. Moreover, Hsp70 and ATP synergistically enhanced the binding of Pex5p to the C-terminal PTS1-containing part of AOx, implying that Pex5p recognizes its cargo PTS1 protein by chaperone-assisted as well as energy-dependent mechanisms in vivo.
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