ArticleThe Biochemical journal2001
Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 79 citations in OpenAlex.
- Human skeletal muscle glycogen utilization in exhaustive exercise: role of subcellular localization and fibre type.The Journal of physiology · 2011Trial
- OGG1 increases exercise endurance via elevated skeletal muscle FGF21.The Journal of biological chemistry · 2026Article
- Active Glycogen Synthase in the Liver Prevents High-Fat Diet-Induced Glucose Intolerance, Decreases Food Intake, and Lowers Body Weight.International journal of molecular sciences · 2023Article
- Principles and functions of metabolic compartmentalization.Nature metabolism · 2022Review
- Antihyperglycemic and antihyperlipidemic activities of wild musk melon (Chinese herbal medicines · 2020Article
- Fibrillar Collagen Type I Participates in the Survival and Aggregation of Primary Hepatocytes Cultured on Soft Hydrogels.Biomimetics (Basel, Switzerland) · 2020Article
- Maintenance of liver glycogen during long-term fasting preserves energy state in mice.FEBS letters · 2020Article
- Brain Glycogen Structure and Its Associated Proteins: Past, Present and Future.Advances in neurobiology · 2019Review
- Biological characterization of a novel hybrid copolymer carrier system based on glycogen.Drug delivery and translational research · 2018Article
- Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels.Scientific reports · 2018Article
- Effects of hepatic glycogen on food intake and glucose homeostasis are mediated by the vagus nerve in mice.Diabetologia · 2017Article
- Genetic models rule out a major role of beta cell glycogen in the control of glucose homeostasis.Diabetologia · 2016Article
- Neuronal glycogen synthesis contributes to physiological aging.Aging cell · 2014Article
- Glucose-6-phosphate-mediated activation of liver glycogen synthase plays a key role in hepatic glycogen synthesis.Diabetes · 2013Article
- The 3T3-L1 adipocyte glycogen proteome.Proteome science · 2013Article
- Brain glycogen-new perspectives on its metabolic function and regulation at the subcellular level.Frontiers in neuroenergetics · 2012Article
- Restoration of hepatic glycogen deposition reduces hyperglycaemia, hyperphagia and gluconeogenic enzymes in a streptozotocin-induced model of diabetes in rats.Diabetologia · 2011Article
- Hepatic overexpression of a constitutively active form of liver glycogen synthase improves glucose homeostasis.The Journal of biological chemistry · 2010Article
- Regulation of glycogen metabolism in yeast and bacteria.FEMS microbiology reviews · 2010Review
- "Fluorescent glycogen" formation with sensibility for in vivo and in vitro detection.Glycoconjugate journal · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in the intracellular distribution of liver glycogen synthase (GS) might constitute a new regulatory mechanism for the activity of this enzyme at cellular level. Our previous studies indicated that incubation of isolated hepatocytes with glucose activated GS and resulted in its translocation from a homogeneous cytosolic distribution to the cell periphery. These studies also suggested a relationship with insoluble elements of the cytoskeleton, in particular actin. Here we show the translocation of GS in a different experimental model that allows the analysis of this phenomenon in long-term studies. We describe the reversibility of translocation of GS and its effect on glycogen distribution. Incubation of cultured rat hepatocytes with glucose activated GS and triggered its translocation to the hepatocyte periphery. The relative amount of the enzyme concentrated near the plasma membrane increased with time up to 8 h of incubation with glucose, when the glycogen stores reached their maximal value. The lithium-induced covalent activation of GS was not sufficient to cause its translocation to the cell periphery. The intracellular distribution of GS closely resembled that of glycogen. Our results showed an interaction between GS and an insoluble element of the hepatocyte matrix. Although no co-localization between actin filaments and GS was observed in any condition, disruption of actin cytoskeleton resulted in a significantly lower percentage of cells in which the enzyme translocated to the cell periphery in response to glucose. This observation suggests that the microfilament network has a role in the translocation of GS.
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