ArticlePlant molecular biology2001
A constitutive cystatin-encoding gene from barley (Icy) responds differentially to abiotic stimuli.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 102 citations in OpenAlex.
- Article
- Expression profile of cystatin gene family in alfalfa (Medicago sativa L.) related to biotic and abiotic stress response.BMC genomics · 2025Article
- Enhancement of broad-spectrum disease resistance in wheat through key genes involved in systemic acquired resistance.Frontiers in plant science · 2024Review
- Genome-Wide Identification and Expression Analysis of the Protease Inhibitor Gene Families in Tomato.Genes · 2019Article
- Expression analysis of proteinase inhibitor-II under3 Biotech · 2018Article
- Article
- Overexpression ofFrontiers in plant science · 2017Article
- Molecular Characterization and Expression Profiling ofFrontiers in plant science · 2017Article
- Identification of drought stress related proteins from 1SBotanical studies · 2016Article
- Epigenomics and bolting tolerance in sugar beet genotypes.Journal of experimental botany · 2016Article
- DREB2C acts as a transcriptional activator of the thermo tolerance-related phytocystatin 4 (AtCYS4) gene.Transgenic research · 2014Article
- Abiotic stress-responsive expression of wali1 and wali5 genes from wheat.Plant signaling & behavior · 2012Article
- Molecular analysis of maize cystatin expression as fusion product in Escherichia coli.Physiology and molecular biology of plants : an international journal of functional plant biology · 2012Article
- Molecular Cloning and Expression Analysis of Triticale Phytocystatins During Development and Germination of Seeds.Plant molecular biology reporter · 2012Article
- iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis.Plant physiology · 2011Article
- Distinct expression patterns of two Arabidopsis phytocystatin genes, AtCYS1 and AtCYS2, during development and abiotic stresses.Plant cell reports · 2010Article
- In silico analysis of sequential, structural and functional diversity of wheat cystatins and its implication in plant defense.Genomics, proteomics & bioinformatics · 2010Article
- Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination.Plant physiology · 2009Article
- Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6.Plant cell reports · 2009Article
- Two cysteine proteinase inhibitors from Arabidopsis thaliana, AtCYSa and AtCYSb, increasing the salt, drought, oxidation and cold tolerance.Plant molecular biology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A barley cDNA clone encoding a cysteine proteinase inhibitor was characterized. The deduced amino acid sequence of this barley cystatin (Hv-CPI) contains the motif QXVXG conserved among members of the cystatin superfamily. The gene (Icy), located on chromosome 2, was expressed in embryos, developing endosperms, leaves and roots as assessed by northern blot analysis. Western blot analysis detected a slightly retarded band in leaves that was not present in roots or seeds. In these two organs a more precise location of Hv-CPI was done by immuno-histochemical analysis, with polyclonal antibodies raised against the recombinant CPI protein expressed in Escherichia coli. This protein efficiently inhibited papain (Ki 2.0 x 10(-8) M) and ficin (Ki 2.2 x 10(-8) M) and, to a lesser extent, chymopapain (Ki 1.6 x 10(-7) M) and was inactive against bromelain. The Icy mRNA expression in vegetative tissues increased in response to anaerobiosis, dark and cold shock (6 degrees C).
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