ArticlePlant molecular biology2001
Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
- Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localisation.Plant biotechnology journal · 2026Article
- Antifungal activity and genome annotation of Bacillus amyloliquefaciens subsp. plantarum SMLR1, a potential biocontrol agent of Rhizoctonia solani.World journal of microbiology & biotechnology · 2025Article
- Shifting Routes: Plant-Specific Insert Trafficking and Function in Arabidopsis Seedlings Under Abiotic Stress.Plant direct · 2025Article
- Aspartic proteases from Silybum marianum: different plant-specific inserts, different destinations.Planta · 2025Article
- Interaction Dynamics of Plant-Specific Insert Domains fromMolecules (Basel, Switzerland) · 2024Article
- Bioactive Compounds from Artichoke and Application Potential.Food technology and biotechnology · 2023Review
- The journey of cardosin A in young Arabidopsis seedlings leads to evidence of a Golgi-independent pathway to the protein storage vacuole.Frontiers in plant science · 2023Article
- Bioactivities of Mealworm (Biology · 2022Article
- Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes.Scientific reports · 2021Article
- Genome-wide identification of the aspartic protease gene family and their response under powdery mildew stress in wheat.Molecular biology reports · 2020Article
- Molecular Properties and New Potentials of Plant Nepenthesins.Plants (Basel, Switzerland) · 2020Review
- Plant Aspartic Proteases for Industrial Applications: Thistle Get Better.Plants (Basel, Switzerland) · 2020Review
- Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L.Plant cell reports · 2020Article
- N-Linked Glycosylation Modulates Golgi-Independent Vacuolar Sorting Mediated by the Plant Specific Insert.Plants (Basel, Switzerland) · 2019Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- Delivering of proteins to the plant vacuole--an update.International journal of molecular sciences · 2014Review
- Proteomics profiling reveals novel proteins and functions of the plant stigma exudate.Journal of experimental botany · 2013Article
- Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape.BMC genomics · 2013Article
- Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii.Planta · 2012Article
- The heterologous systems in the study of cardosin B trafficking pathways.Plant signaling & behavior · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cardosins A and B are related aspartic proteinases from the pistils of Cynara cardunculus L., whose milk-clotting activity has been exploited for the manufacture of cheese. Here we report the cloning of cardosin B cDNA and its organ, tissue and cytological localization. The cDNA-derived amino acid sequence has 73% similarity with that of cardosin A and displays several distinguishing features. Cardosin B mRNA was detected in young inflorescences but not in pistils of fully opened inflorescences, indicating that its expression is developmentally regulated. The proteinase, however, accumulates in the pistil until the later stages of floral development. Immunocytochemistry with a monospecific antibody localized cardosin B to the cell wall and extracellular matrix of the floral transmitting tissue. The location of cardosin B in the pistil is therefore clearly different from that of cardosin A, which was found at protein storage vacuoles of the stigmatic papillae and has been suggested to be involved in RGD-mediated proteolytic mechanisms. In view of these results the possible functions of cardosin B in the transmitting tissue are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.