ArticleJournal of virology2001
Role of the 3'-untranslated regions of alfalfa mosaic virus RNAs in the formation of a transiently expressed replicase in plants and in the assembly of virions.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Binding between elongation factor 1A and the 3'-UTR of Chinese wheat mosaic virus is crucial for virus infection.Molecular plant pathology · 2021Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Activation of Tomato Bushy Stunt Virus RNA-Dependent RNA Polymerase by Cellular Heat Shock Protein 70 Is Enhanced by Phospholipids In Vitro.Journal of virology · 2015Article
- Article
- p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract.Journal of virology · 2012Article
- Identification and characterization of the 480-kilodalton template-specific RNA-dependent RNA polymerase complex of red clover necrotic mosaic virus.Journal of virology · 2010Article
- In vitro and in vivo studies of the RNA conformational switch in Alfalfa mosaic virus.Journal of virology · 2010Article
- Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus.Advances in virus research · 2010Review
- Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast.Journal of virology · 2008Article
- Alfalfa mosaic virus coat protein bridges RNA and RNA-dependent RNA polymerase in vitro.Virology · 2007Article
- Article
- Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis.RNA (New York, N.Y.) · 2007Article
- Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.Journal of virology · 2005Article
- Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase.Journal of virology · 2005Article
- Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity.Journal of virology · 2004Article
- Biased hypermutagenesis associated with mutations in an untranslated hairpin of an RNA virus.Journal of virology · 2004Article
- tRNA-like structure regulates translation of Brome mosaic virus RNA.Journal of virology · 2004Article
- Coordinate replication of alfalfa mosaic virus RNAs 1 and 2 involves cis- and trans-acting functions of the encoded helicase-like and polymerase-like domains.Journal of virology · 2003Article
- The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis.Journal of virology · 2003Article
- Role of the alfalfa mosaic virus methyltransferase-like domain in negative-strand RNA synthesis.Journal of virology · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa mosaic virus (AMV) RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively, whereas RNA 3 encodes the movement protein and the coat protein (CP). When RNAs 1 and 2 were transiently expressed from a T-DNA vector (R12 construct) by agroinfiltration of Nicotiana benthamiana, the infiltrated leaves accumulated minus-strand RNAs 1 and 2 and relatively small amounts of plus-strand RNAs. In addition, RNA-dependent RNA polymerase (RdRp) activity could be detected in extracts of the infiltrated leaves. After transient expression of RNAs 1 and 2 with the 3'-untranslated regions (UTRs) of both RNAs deleted (R1Delta/2Delta construct), no replication of RNAs 1 and 2 was observed, while the infiltrated leaves supported replication of RNA 3 after inoculation of the leaves with RNA 3 or expression of RNA 3 from a T-DNA vector (R3 construct). No RdRp activity could be isolated from leaves infiltrated with the R1Delta/2Delta construct, although P1 and P2 sedimented in a region of a glycerol gradient where active RdRp was found in plants infiltrated with R12. RdRp activity could be isolated from leaves infiltrated with constructs R1Delta/2 (3'-UTR of RNA 1 deleted), R1/2Delta (3'-UTR of RNA 2 deleted), or R1Delta/2Delta plus R3. This demonstrates that the 3'-UTR of AMV RNAs is required for the formation of a complex with in vitro enzyme activity. RNAs 1 and 2 with the 3'-UTRs deleted were encapsidated into virions by CP expressed from RNA 3. This shows that the high-affinity binding site for CP at the 3'-termini of AMV RNAs is not required for assembly of virus particles.
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