ArticleJournal of virology2001
Multiple cis regulatory elements control RANTES promoter activity in alveolar epithelial cells infected with respiratory syncytial virus.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 98 citations in OpenAlex.
- Article
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- MEK inhibition drives anti-viral defence in RV but not RSV challenged human airway epithelial cells through AKT/p70S6K/4E-BP1 signalling.Cell communication and signaling : CCS · 2019Article
- Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway.Journal of neuroinflammation · 2016Article
- Role of hydrogen sulfide in paramyxovirus infections.Journal of virology · 2015Article
- Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.Nature communications · 2014Article
- Interferon regulatory factor 6 differentially regulates Toll-like receptor 2-dependent chemokine gene expression in epithelial cells.The Journal of biological chemistry · 2014Article
- SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- HIV-1 Nef induces CCL5 production in astrocytes through p38-MAPK and PI3K/Akt pathway and utilizes NF-kB, CEBP and AP-1 transcription factors.Scientific reports · 2014Article
- Human immunodeficiency virus type 1 viral protein R (Vpr) induces CCL5 expression in astrocytes via PI3K and MAPK signaling pathways.Journal of neuroinflammation · 2013Article
- Respiratory syncytial virus infection: mechanisms of redox control and novel therapeutic opportunities.Antioxidants & redox signaling · 2013Review
- Human metapneumovirus glycoprotein G disrupts mitochondrial signaling in airway epithelial cells.PloS one · 2013Article
- Dendritic cells activated by IFN-γ/STAT1 express IL-31 receptor and release proinflammatory mediators upon IL-31 treatment.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis.BMC genomics · 2012Article
- TAK1 regulates NF-ΚB and AP-1 activation in airway epithelial cells following RSV infection.Virology · 2011Article
- Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells.Virus genes · 2011Article
- Respiratory syncytial virus pathophysiology and affect of palivizumab in special populations: cystic fibrosis and immunosuppression.The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG · 2011Article
- Human metapneumovirus inhibits IFN-β signaling by downregulating Jak1 and Tyk2 cellular levels.PloS one · 2011Article
- Article
- Pharmacologic advances in the treatment and prevention of respiratory syncytial virus.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2010Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Respiratory syncytial virus (RSV) produces intense pulmonary inflammation, in part through its ability to induce chemokine synthesis in infected airway epithelial cells. RANTES (regulated upon activation, normally T-cell expressed and presumably secreted) is a CC chemokine which recruits and activates monocytes, lymphocytes, and eosinophils, all cell types present in the lung inflammatory infiltrate induced by RSV infection. In this study, we analyzed the mechanism of RSV-induced RANTES promoter activation in human type II alveolar epithelial cells (A549 cells). Promoter deletion and mutagenesis experiments indicate that RSV requires the presence of five different cis regulatory elements, located in the promoter fragment spanning from -220 to +55 nucleotides, corresponding to NF-kappaB, C/EBP, Jun/CREB/ATF, and interferon regulatory factor (IRF) binding sites. Although site mutations of the NF-kappaB, C/EBP, and CREB/AP-1 like sites reduce RSV-induced RANTES gene transcription to 50% or less, only mutations affecting IRF binding completely abolish RANTES inducibility. Supershift and microaffinity isolation assays were used to identify the different transcription factor family members whose DNA binding activity was RSV inducible. Expression of dominant negative mutants of these transcription factors further established their central role in virus-induced RANTES promoter activation. Our finding that the presence of multiple cis regulatory elements is required for full activation of the RANTES promoter in RSV-infected alveolar epithelial cells supports the enhanceosome model for RANTES gene transcription, which is absolutely dependent on binding of IRF transcription factors. The identification of regulatory mechanisms of RANTES gene expression is fundamental for rational design of inhibitors of RSV-induced lung inflammation.
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