ArticleJournal of virology2001
RNA recombination between persisting pestivirus and a vaccine strain: generation of cytopathogenic virus and induction of lethal disease.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Live Attenuated Vaccines for African Swine Fever: Perspectives on Their Use and Strategic Directions.Transboundary and emerging diseases · 2026Review
- Self-Replicating RNA Derived from the Genomes of Positive-Strand RNA Viruses.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Immune protection induced by E2 recombinant glycoprotein of bovine viral diarrhea virus in a murine model.Frontiers in veterinary science · 2023Article
- Characterization of a Cytopathogenic Reporter CSFV.Viruses · 2021Article
- Bovine Pestivirus Heterogeneity and Its Potential Impact on Vaccination and Diagnosis.Viruses · 2020Review
- Senecavirus-Specific Recombination Assays Reveal the Intimate Link between Polymerase Fidelity and RNA Recombination.Journal of virology · 2019Article
- CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses.Journal of virology · 2019Article
- Factors Affecting the Tissue Damaging Consequences of Viral Infections.Frontiers in microbiology · 2019Review
- Virological and Immunological Outcomes of Coinfections.Clinical microbiology reviews · 2018Review
- Self-Replicating RNA.Methods in molecular biology (Clifton, N.J.) · 2017Review
- Review
- Evolution of infectious bronchitis virus in China over the past two decades.The Journal of general virology · 2016Article
- Recombination of Globally Circulating Varicella-Zoster Virus.Journal of virology · 2015Article
- Hepatitis C virus genetic variability and evolution.World journal of hepatology · 2015Review
- Evolution and Emergence of Enteroviruses through Intra- and Inter-species Recombination: Plasticity and Phenotypic Impact of Modular Genetic Exchanges in the 5' Untranslated Region.PLoS pathogens · 2015Article
- Cell type-dependent RNA recombination frequency in the Japanese encephalitis virus.BioMed research international · 2014Article
- Article
- Productive homologous and non-homologous recombination of hepatitis C virus in cell culture.PLoS pathogens · 2013Article
- Article
- RNA structural elements determine frequency and sites of nonhomologous recombination in an animal plus-strand RNA virus.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular analysis of a cytopathogenic (cp) bovine viral diarrhea virus (BVDV) isolate (1741) obtained from a case of mucosal disease (MD) led to the identification of five different viral subgenomic RNAs in addition to a noncytopathogenic (noncp) strain (NCP 1741). For each of the subgenomes, a large internal deletion was found together with an inserted sequence encoding part of ribosomal protein S27a fused to an N-terminally truncated ubiquitin monomer. Surprisingly, the two cellular insertions together with flanking viral sequences encoding parts of NS3 and NS4B are >99% identical to the previously described sequence of BVDV vaccine strain RIT (P. Becher, M. Orlich, and H.-J. Thiel, J. Virol. 72:8697-8704, 1998), while the remainder of the subgenomes is derived from the genome of NCP 1741. Further analyses including molecular cloning and nucleotide sequencing of the recombination partners revealed that both homologous and nonhomologous RNA recombination contributed to the generation of the viral subgenomes. Interestingly, for another cp BVDV isolate (CP 4584) from an independent case of MD, again an insertion of a RIT-derived sequence element was detected. In contrast to CP 1741, for CP 4584 a duplication of the genomic region encoding NS3 and parts of NS4A and NS4B was found. Transfection of bovine cells with RNA transcribed from a chimeric cDNA construct showed that the RIT-derived insertion together with the CP 4584-specific duplication of viral sequences represents the genetic basis of cytopathogenicity of CP 4584. Remarkably, passages of the recovered cp virus in cell culture led to emergence of noncp BVDV and a number of viral subgenomes whose genome organization was similar to that in BVDV 1741.
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