ArticleImmunology2001
The role of the macrophage scavenger receptor in immune stimulation by bacterial DNA and synthetic oligonucleotides.
Article in Immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Article
- Article
- Transmission of trained immunity and heterologous resistance to infections across generations.Nature immunology · 2021Article
- Rapid Complexation of Aptamers by Their Specific Antidotes.Molecules (Basel, Switzerland) · 2017Article
- Strategies and hurdles using DNA vaccines to fish.Veterinary research · 2014Review
- SR-A regulates the inflammatory activation of astrocytes.Neurotoxicity research · 2014Article
- Nucleic acids and endosomal pattern recognition: how to tell friend from foe?Frontiers in cellular and infection microbiology · 2013Review
- Traditional biochemical assays for studying toll-like receptor 9.Journal of immunoassay & immunochemistry · 2013Review
- Scavenger receptors target glycolipids for natural killer T cell activation.The Journal of clinical investigation · 2012Article
- Scavenger receptor A (SR-A) is required for LPS-induced TLR4 mediated NF-κB activation in macrophages.Biochimica et biophysica acta · 2012Article
- Article
- Toll-like receptors: new players in myocardial ischemia/reperfusion injury.Antioxidants & redox signaling · 2011Review
- Scavenger receptor class-A has a central role in cerebral ischemia-reperfusion injury.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2010Article
- PolyI:C-induced reduction in uptake of soluble antigen is independent of dendritic cell activation.International immunology · 2009Article
- The macrophage scavenger receptor A is host-protective in experimental meningococcal septicaemia.PLoS pathogens · 2009Article
- SR-A, MARCO and TLRs differentially recognise selected surface proteins from Neisseria meningitidis: an example of fine specificity in microbial ligand recognition by innate immune receptors.Journal of innate immunity · 2009Article
- Macrophage pattern recognition receptors in immunity, homeostasis and self tolerance.Advances in experimental medicine and biology · 2009Review
- Receptor-mediated endocytosis of phosphodiester oligonucleotides in the HepG2 cell line: evidence for non-conventional intracellular trafficking.Nucleic acids research · 2002Article
- Anatomical site-specific contributions of pneumococcal virulence determinants.Pneumonia (Nathan Qld.)Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To assess the role of the macrophage scavenger receptor type A (SRA) in immune activation by CpG DNA, cytokine induction and DNA uptake were tested in vitro and in vivo using SRA knockout (SRA-/-) and wild type (WT) mice. As a source of CpG DNA, Escherichia coli DNA (EC DNA) and a 20-mer phosphorothioate oligodeoxynucleotide with two CpG motifs (CpG ODN) were used. In vitro, both EC DNA and the CpG ODN induced dose-dependent increases of interleukin (IL)-12 production by spleen cells and bone-marrow-derived macrophages (BMMPhi) from both SRA-/- and WT mice. The levels of cytokines produced by SRA-/- spleen cells and BMMPhi were similar to those of WT spleen cells and BMMPhi. When injected intravenously with CpG ODN and EC DNA, both SRA-/- and WT mice showed elevated serum levels of IL-12. To investigate further the role of the SRA, flow cytometry and confocal microscopy were performed to examine the uptake of fluorescently labelled oligonucleotides. SRA-/- and WT BMMPhi showed similarity in the extent of uptake and distribution of oligonucleotides as assessed by these two techniques. Together, these findings indicate that, while the SRA may bind DNA, this receptor is not essential for the uptake of CpG DNA or its immunostimulatory activity.
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Registered trials
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