ArticleNucleic acids research2001
Gene expression in isolated plant mitochondria: high fidelity of transcription, splicing and editing of a transgene product in electroporated organelles.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria.Nature communications · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Applications of CPPs in Genome Editing of Plants.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Molecular and Functional Diversity of RNA Editing in Plant Mitochondria.Molecular biotechnology · 2018Review
- Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues.Plant methods · 2017Article
- Article
- Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers.Scientific reports · 2015Article
- Deletions in cox2 mRNA result in loss of splicing and RNA editing and gain of novel RNA editing sites.PloS one · 2013Article
- Article
- A protein shuttle system to target RNA into mitochondria.Nucleic acids research · 2011Article
- Mitochondrial mRNA polymorphisms in different Arabidopsis accessions.Plant physiology · 2008Article
- RNA editing site recognition in heterologous plant mitochondria.Current genetics · 2006Article
- An in vitro RNA editing system from cauliflower mitochondria: editing site recognition parameters can vary in different plant species.RNA (New York, N.Y.) · 2005Article
- Mitochondrial electroporation and in organello RNA editing of chimeric atp6 transcripts.Molecular genetics and genomics : MGG · 2005Article
- Gene expression studies in isolated mitochondria: Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription, splicing and editing machinery of wheat mitochondria.Nucleic acids research · 2005Article
- Gamma carbonic anhydrases in plant mitochondria.Plant molecular biology · 2004Article
- The fertility restorer genes X and T alter the transcripts of a novel mitochondrial gene implicated in CMS1 in chives (Allium schoenoprasum L.).Molecular genetics and genomics : MGG · 2004Article
- Different patterns in the recognition of editing sites in plant mitochondria.Nucleic acids research · 2004Article
- Complex cis-elements determine an RNA editing site in pea mitochondria.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial gene expression was studied using an electrotransformation protocol to introduce foreign DNA into purified wheat mitochondria. Optimal conditions for DNA uptake and transient gene expression were determined. We show here that a DNA plasmid containing either a cognate or a non-cognate gene under the control of a plant mitochondrial promoter is incorporated into the organelle and faithfully recognized by the transcription machinery. Transcripts generated by a plasmid bearing the intron-containing cox II gene were correctly spliced. Moreover, the transcripts were edited at the expected target C residues. The expression and maturation process of the transgene is dependent on the integrity of functional elements such as the promotor or the presence of structural domains necessary for splicing. The mitochondrial transformation described in this report is an important tool to study the multiple steps involved in plant mitochondrial gene expression at conditions closer to those found in vivo.
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