ArticleThe Journal of physiology2001
Free intracellular Mg(2+) concentration and inhibition of NMDA responses in cultured rat neurons.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
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- Neuronal acid-induced [Zn²⁺]i elevations calibrated using the low-affinity ratiometric probe FuraZin-1.Journal of neurochemistry · 2015Article
- Functional study of NIPA2 mutations identified from the patients with childhood absence epilepsy.PloS one · 2014Article
- Mechanistic and structural determinants of NMDA receptor voltage-dependent gating and slow Mg2+ unblock.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- New drug targets in depression: inflammatory, cell-mediated immune, oxidative and nitrosative stress, mitochondrial, antioxidant, and neuroprogressive pathways. And new drug candidates--Nrf2 activators and GSK-3 inhibitors.Inflammopharmacology · 2012Review
- Effects of exogenous hydrogen sulphide on calcium signalling, background (TASK) K channel activity and mitochondrial function in chemoreceptor cells.Pflugers Archiv : European journal of physiology · 2012Article
- Control of neuronal excitability by calcium binding proteins: a new mathematical model for striatal fast-spiking interneurons.Frontiers in molecular neuroscience · 2012Article
- Relief of Mg²⁺-dependent inhibition of TRPM1 by PKCα at the rod bipolar cell synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Deconstructing GSK-3: The Fine Regulation of Its Activity.International journal of Alzheimer's disease · 2011Article
- Control of local intracellular calcium concentration with dynamic-clamp controlled 2-photon uncaging.PloS one · 2011Article
- Voltage-dependent gating of NR1/2B NMDA receptors.The Journal of physiology · 2008Article
- Kinetic properties of DM-nitrophen binding to calcium and magnesium.Biophysical journal · 2005Article
- Amantadine inhibits NMDA receptors by accelerating channel closure during channel block.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Developmental changes in parvalbumin regulate presynaptic Ca2+ signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Action potential timing determines dendritic calcium during striatal up-states.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- 'New' functions for 'old' proteins: the role of the calcium-binding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology. Studies with knockout mice.Cerebellum (London, England) · 2002Review
- Modulation by permeant ions of Mg(2+) inhibition of NMDA-activated whole-cell currents in rat cortical neurons.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. Intracellular Mg(2+) (Mg(2+)(i)) blocks single-channel currents and modulates the gating kinetics of NMDA receptors. However, previous data suggested that Mg(2+)(i) inhibits whole-cell current less effectively than predicted from excised-patch measurements. We examined the basis of this discrepancy by testing three hypothetical explanations. 2. To test the first hypothesis, that control of free Mg(2+)(i) concentration ([Mg(2+)](i)) during whole-cell recording was inadequate, we measured [Mg(2+)](i) using mag-indo-1 microfluorometry. The [Mg(2+)](i) measured in cultured neurons during whole-cell recording was similar to the pipette [Mg(2+)] measured in vitro, suggesting that [Mg(2+)](i) was adequately controlled. 3. To test the second hypothesis, that open-channel block by Mg(2+)(i) was modified by patch excision, we characterised the effects of Mg(2+)(i) using cell-attached recordings. We found the affinity and voltage dependence of open-channel block by Mg(2+)(i) similar in cell-attached and outside-out patches. Thus, the difference between Mg(2+)(i) inhibition of whole-cell and of patch currents cannot be attributed to a difference in Mg(2+)(i) block of single-channel current. 4. The third hypothesis tested was that the effect of Mg(2+)(i) on channel gating was modified by patch excision. Results of cell-attached recording and modelling of whole-cell data suggest that the Mg(2+)(i)-induced stabilisation of the channel open state is four times weaker after patch excision than in intact cells. This differential effect of Mg(2+)(i) on channel gating explains why Mg(2+)(i) inhibits whole-cell NMDA responses less effectively than patch responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.