ArticleThe Journal of physiology2001
Glia-derived signals induce synapse formation in neurones of the rat central nervous system.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- The Role of Kinase Inhibitors in Cancer Neuroscience: Mechanisms, Therapeutic Potential, and Future Directions.Anti-cancer agents in medicinal chemistry · 2026Review
- Optimization of an autaptic culture system for studying cholinergic synapses in sympathetic ganglia.Pflugers Archiv : European journal of physiology · 2025Article
- Astrocytes in the adult dentate gyrus-balance between adult and developmental tasks.Molecular psychiatry · 2024Review
- Glial fibrillary acidic protein in Alzheimer's disease: a narrative review.Brain communications · 2024Review
- The normalizing effects of the CYP46A1 activator efavirenz on retinal sterol levels and risk factors for glaucoma in ApojCellular and molecular life sciences : CMLS · 2023Article
- The neuroscience of cancer.Nature · 2023Review
- Cholesterol and oxysterols in retinal neuron-glia interactions: relevance for glaucoma.Frontiers in ophthalmology · 2023Review
- Multivesicular release favors short term synaptic depression in hippocampal autapses.Frontiers in cellular neuroscience · 2023Article
- The heterogeneity of astrocytes in glaucoma.Frontiers in neuroanatomy · 2022Review
- Astrocytes in cocaine addiction and beyond.Molecular psychiatry · 2022Review
- Cholesterol metabolism and brain injury in neonatal encephalopathy.Pediatric research · 2021Review
- Ketamine Alters Functional Plasticity of Astroglia: An Implication for Antidepressant Effect.Life (Basel, Switzerland) · 2021Review
- Neuron-Oligodendrocyte Communication in Myelination of Cortical GABAergic Cells.Life (Basel, Switzerland) · 2021Review
- The role of astrocyte-mediated plasticity in neural circuit development and function.Neural development · 2021Review
- Ketamine Action on Astrocytes Provides New Insights into Rapid Antidepressant Mechanisms.Advances in neurobiology · 2021Review
- Astrocyte-Neuron Signaling in Synaptogenesis.Frontiers in cell and developmental biology · 2021Review
- Activity Shapes Neural Circuit Form and Function: A Historical Perspective.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Review
- Dysregulated Lipid Metabolism and Its Role in α-Synucleinopathy in Parkinson's Disease.Frontiers in neuroscience · 2019Review
- Cell Biology of Astrocyte-Synapse Interactions.Neuron · 2017Review
- Roles of Glial Cells in Sculpting Inhibitory Synapses and Neural Circuits.Frontiers in molecular neuroscience · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. To study the effects of glial cells on synapse formation, we established microcultures of purified rat retinal ganglion cells (RGCs) and monitored synapse (autapse) development in single neurones using electrophysiological recordings, FM1-43 labelling and immunocytochemistry. 2. Solitary neurones grew ramifying neurites, but formed only very few and inefficient excitatory autapses, when cultured for up to 2 weeks in defined medium and in the absence of glial cells. 3. Treatment of glia-free microcultures of RGCs with glia-conditioned medium (GCM) increased the number of autapses per neurone by up to 10-fold. This was indicated by a similar increase in the frequency of spontaneous events and the number of FM1-43-labelled functional release sites and of puncta, where pre- and postsynaptic markers colocalized. 4. In addition, GCM treatment enhanced the efficacy of presynaptic transmitter release as indicated by lower failure rates of stimulation-induced excitatory autaptic currents, a 200-fold increase in the frequency of asynchronous release and an accelerated stimulation-induced FM1-43 destaining. Furthermore, GCM induced an increase in the quantal size. 5. GCM affected autaptic activity not immediately, but with a delay of 24 h, and the effects on stimulation-induced autaptic currents occurred before changes in the frequency of spontaneous events indicating an early strengthening of existing autapses followed by a later increase in autapse number. 6. The observed effects were mediated by proteinase K-sensitive factors in GCM and occurred independently of electrical activity. 7. These results suggest that soluble glia-derived signals induce synapse formation and maturation in neurones of the central nervous system (CNS).
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