ArticleGenes & development2001
Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces.
Article in Genes & development, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 116 citations in OpenAlex.
- Taming Mre11-Rad50 at Telomeres: Convergent Strategies for Chromosome-End Protection Across Evolution.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Engineering yeast chromosomal telomeres with a bacteriophage system.Nature communications · 2026Article
- Giant linear plasmids in Mycobacterium avium harbour a tRNA array unit.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Phage-encoded TelN inhibits bacterial Mre11-Rad50 nuclease to protect hairpin telomeres.The EMBO journal · 2025Article
- Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation.Environmental microbiology · 2025Article
- Origin, Evolution and Diversity of φ29-like Phages-Review and Bioinformatic Analysis.International journal of molecular sciences · 2024Review
- Article
- Dissection of 3D chromosome organization inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Extremophile Metal Resistance: Plasmid-Encoded Functions in Streptomyces mirabilis.Applied and environmental microbiology · 2022Article
- Telomeric and sub-telomeric regions undergo rapid turnover within a Streptomyces population.Scientific reports · 2020Article
- Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 EncodingFrontiers in microbiology · 2019Article
- Telomere associated primase Tap repairs truncated telomeres of Streptomyces.Nucleic acids research · 2017Article
- DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication.Frontiers in molecular biosciences · 2016Review
- Telomere-associated proteins add deoxynucleotides to terminal proteins during replication of the telomeres of linear chromosomes and plasmids in Streptomyces.Nucleic acids research · 2015Article
- The flagellar set Fla2 in Rhodobacter sphaeroides is controlled by the CckA pathway and is repressed by organic acids and the expression of Fla1.Journal of bacteriology · 2015Article
- Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy.Frontiers in microbiology · 2015Review
- A distant homologue of the FlgT protein interacts with MotB and FliL and is essential for flagellar rotation in Rhodobacter sphaeroides.Journal of bacteriology · 2013Article
- Mutational analysis of the terminal protein Tpg of Streptomyces chromosomes: identification of the deoxynucleotidylation site.PloS one · 2013Article
- A novel component of the Rhodobacter sphaeroides Fla1 flagellum is essential for motor rotation.Journal of bacteriology · 2012Article
- Translesion-synthesis DNA polymerases participate in replication of the telomeres in Streptomyces.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Linear plasmids and chromosomes of the bacterial genus Streptomyces have proteins of unknown characteristics and function linked covalently to their 5' DNA termini. We purified protein attached to the end of the pSLA2 linear plasmid of Streptomyces rochei, determined the N-terminal amino acid sequence, and used this information to clone corresponding genes from a S. rochei cosmid library. Three separate terminal protein genes (here designated as tpgR1, tpgR2, and tpgR3), which map to the S. rochei chromosome and to 100-kb and 206-kb linear plasmids contained in S. rochei, were isolated and found to encode a family of similar but distinct 21-kD proteins. Using tpgR1 to probe a genomic DNA library of Streptomyces lividans ZX7, whose linear chromosome can undergo transition to a circular form, we isolated a S. lividans chromosomal gene (tpgL) that we found specifies a protein closely related to, and functionally interchangeable with, TpgR proteins for pSLA2 maintenance in S. lividans. Mutation of tpgL precluded propagation of the pSLA2 plasmid in a linear form and also prevented propagation of S. lividans cells that contain linear, but not circular, chromosomes, indicating a specific and essential role for tpg genes in linear DNA replication. Surprisingly, Tpg proteins were observed to contain a reverse transcriptase-like domain rather than sequences in common with proteins that attach covalently to the termini of linear DNA replicons.
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