ArticleMolecular biology of the cell2001
Homotypic fusion of immature secretory granules during maturation requires syntaxin 6.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 128 citations in OpenAlex.
- Cdc42 coordinates apical membrane formation and secretory granule maturation through v-SNARE maintenance in salivary acinar cells.The Journal of biological chemistry · 2026Article
- A defined clathrin-mediated trafficking pathway regulates sFLT1/VEGFR1 secretion from endothelial cells.Angiogenesis · 2024Article
- A defined clathrin-mediated trafficking pathway regulates sFLT1/VEGFR1 secretion from endothelial cells.bioRxiv : the preprint server for biology · 2023Article
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Developmental program-independent secretory granule degradation in larval salivary gland cells of Drosophila.Traffic (Copenhagen, Denmark) · 2022Article
- VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.The Journal of cell biology · 2022Article
- A novel function for Rab1 and Rab11 during secretory granule maturation.Journal of cell science · 2021Article
- Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons.Scientific reports · 2020Article
- Cdc42 controls secretory granules morphology in rodent salivary glands in vivo.Communicative & integrative biology · 2020Article
- Syntaxin 3, but not syntaxin 4, is required for mast cell-regulated exocytosis, where it plays a primary role mediating compound exocytosis.The Journal of biological chemistry · 2019Article
- A molecular switch orchestrates enzyme specificity and secretory granule morphology.Nature communications · 2018Article
- Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.Nature communications · 2018Article
- Proteomic analysis of insulin secretory granules in INS-1 cells by protein correlation profiling.Biophysics reports · 2018Article
- Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis.Scientific reports · 2017Article
- The dense-core vesicle maturation protein CCCP-1 binds RAB-2 and membranes through its C-terminal domain.Traffic (Copenhagen, Denmark) · 2017Article
- Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.Scientific reports · 2017Article
- HID-1 is required for homotypic fusion of immature secretory granules during maturation.eLife · 2016Article
- The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.PLoS genetics · 2016Article
- Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland.Journal of visualized experiments : JoVE · 2015Article
- An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells.Biophysics reports · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homotypic fusion of immature secretory granules (ISGs) gives rise to mature secretory granules (MSGs), the storage compartment in endocrine and neuroendocrine cells for hormones and neuropeptides. With the use of a cell-free fusion assay, we investigated which soluble N-ethylmaleimide-sensitive fusion protein attachment receptor (SNARE) molecules are involved in the homotypic fusion of ISGs. Interestingly, the SNARE molecules mediating the exocytosis of MSGs in neuroendocrine cells, syntaxin 1, SNAP-25, and VAMP2, were not involved in homotypic ISG fusion. Instead, we have identified syntaxin 6 as a component of the core machinery responsible for homotypic ISG fusion. Subcellular fractionation studies and indirect immunofluorescence microscopy show that syntaxin 6 is sorted away during the maturation of ISGs to MSGs. Although, syntaxin 6 on ISG membranes is associated with SNAP-25 and SNAP-29/GS32, we could not find evidence that these target (t)-SNARE molecules are involved in homotypic ISG fusion. Nor could we find any involvement for the vesicle (v)-SNARE VAMP4, which is known to be associated with syntaxin 6. Importantly, we have shown that homotypic fusion requires the function of syntaxin 6 on both donor as well as acceptor membranes, which suggests that t-t-SNARE interactions, either direct or indirect, may be required during fusion of ISG membranes.
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