ArticleMolecular biology of the cell2001
Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 176 citations in OpenAlex.
- Centriole Duplication, Maturation, and Transformation During Mammalian Spermatogenesis.Advances in experimental medicine and biology · 2026Review
- Regulation of Xvelo assembly by centrosomal proteins.iScience · 2025Article
- Review
- Oxidative and Molecular-Structural Alterations of Spermatozoa in Swine and Ram Exposed to the Triazole Ipconazole.Toxics · 2025Article
- Article
- Dual-color live imaging unveils stepwise organization of multiple basal body arrays by cytoskeletons.EMBO reports · 2024Article
- Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development.eLife · 2023Article
- The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.The Journal of cell biology · 2022Article
- The apical ciliary adhesion complex is established at the basal foot of motile cilia and depends on the microtubule network.Scientific reports · 2022Article
- Stem cells' centrosomes: How can organelles identified 130 years ago contribute to the future of regenerative medicine?World journal of stem cells · 2021Review
- Review
- Planar cell polarity induces local microtubule bundling for coordinated ciliary beating.The Journal of cell biology · 2021Article
- A centriole's subdistal appendages: contributions to cell division, ciliogenesis and differentiation.Open biology · 2021Review
- Centrosome-centric view of asymmetric stem cell division.Open biology · 2021Review
- Article
- A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids.Cell and tissue research · 2020Article
- The novel testicular enrichment protein Cfap58 is required for Notch-associated ciliogenesis.Bioscience reports · 2020Article
- Genetic and molecular biology of breast cancer among Iranian patients.Journal of translational medicine · 2019Review
- CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton.Frontiers in cell and developmental biology · 2019Article
- Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.Molecular biology of the cell · 2018Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Because centrosomes were enriched in the bile canaliculi fraction from the chicken liver through their association with apical membranes, we developed a procedure for isolation of centrosomes from this fraction. With the use of the centrosomes, we generated centrosome-specific monoclonal antibodies. Three of the monoclonal antibodies recognized an antigen of ~90 kDa. Cloning of its cDNA identified this antigen as a chicken homologue of outer dense fiber 2 protein (Odf2), which was initially identified as a sperm outer dense fiber-specific component. Exogenously expressed and endogenous Odf2 were shown to be concentrated at the centrosomes in a microtubule-independent manner in various types of cells at both light and electron microscopic levels. Odf2 exhibited a cell cycle-dependent pattern of localization and was preferentially associated with the mother centrioles in G0/G1-phase. Toward G1/S-phase before centrosome duplication, it became detectable in both mother and daughter centrioles. In the isolated bile canaliculi and centrosomes, Odf2, in contrast to other centrosomal components, was highly resistant to KI extraction. These findings indicate that Odf2 is a widespread KI-insoluble scaffold component of the centrosome matrix, which may be involved in the maturation event of daughter centrioles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.