ArticleThe Journal of biological chemistry2001
Gln3p nuclear localization and interaction with Ure2p in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 81 citations in OpenAlex.
- Disordered sequences of transcription factors regulate genomic binding by integrating diverse sequence grammars and interaction types.Nucleic acids research · 2024Article
- Effects of abolishing Whi2 on the proteome and nitrogen catabolite repression-sensitive protein production.G3 (Bethesda, Md.) · 2022Article
- Analysis of the phosphorylome of trichoderma reesei cultivated on sugarcane bagasse suggests post-translational regulation of the secreted glycosyl hydrolase Cel7A.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Investigating the role of the transcriptional regulator Ure2 on the metabolism of Saccharomyces cerevisiae: a multi-omics approach.Applied microbiology and biotechnology · 2021Article
- N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization.Genetics · 2021Article
- Sensing, Uptake and Catabolism of L-Phenylalanine During 2-Phenylethanol BiosynthesisFrontiers in microbiology · 2021Review
- Sit4 and PP2A Dephosphorylate Nitrogen Catabolite Repression-Sensitive Gln3 When TorC1 Is Up- as Well as Downregulated.Genetics · 2019Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- More than One Way in: Three Gln3 Sequences Required To Relieve Negative Ure2 Regulation and Support Nuclear Gln3 Import inGenetics · 2018Article
- Regulation of Autophagy through TORC1 and mTORC1.Biomolecules · 2017Review
- Achieving global perfect homeostasis through transporter regulation.PLoS computational biology · 2017Article
- Article
- Multiple Targets on the Gln3 Transcription Activator Are Cumulatively Required for Control of Its Cytoplasmic Sequestration.G3 (Bethesda, Md.) · 2016Article
- Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica.BMC genomics · 2016Article
- The modification of Gat1p in nitrogen catabolite repression to enhance non-preferred nitrogen utilization in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Nuclear Gln3 Import Is Regulated by Nitrogen Catabolite Repression Whereas Export Is Specifically Regulated by Glutamine.Genetics · 2015Article
- Swa2, the yeast homolog of mammalian auxilin, is specifically required for the propagation of the prion variant [URE3-1].Molecular microbiology · 2015Article
- GATA Factor Regulation in Excess Nitrogen Occurs Independently of Gtr-Ego Complex-Dependent TorC1 Activation.G3 (Bethesda, Md.) · 2015Article
- Metabolic engineering of the regulators in nitrogen catabolite repression to reduce the production of ethyl carbamate in a model rice wine system.Applied and environmental microbiology · 2014Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Gln3p is one of two well characterized GATA family transcriptional activation factors whose function is regulated by the nitrogen supply of the cell. When nitrogen is limiting, Gln3p and Gat1p are concentrated in the nucleus where they bind GATA sequences upstream of nitrogen catabolite repression (NCR)-sensitive genes and activate their transcription. Conversely, in excess nitrogen, these GATA sequences are unoccupied by Gln3p and Gat1p because these transcription activators are excluded from the nucleus. Ure2p binds to Gln3p and Gat1p and is required for NCR-sensitive transcription to be repressed and for nuclear exclusion of these transcription factors. Here we show the following. (i) Gln3p residues 344-365 are required for nuclear localization. (ii) Replacing Ser-344, Ser-347, and Ser-355 with alanines has minimal effects on GFP-Gln3p localization. However, replacing Gln3p Ser-344, Ser-347, and Ser-355 with aspartates results in significant loss of its ability to be concentrated in the nucleus. (iii) N and C termini of the Gln3p region required for it to complex with Ure2p and be excluded from the nucleus are between residues 1-103 and 301-365, respectively. (iv) N and C termini of the Ure2p region required for it to interact with Gln3p are situated between residues 101-151 and 330-346, respectively. (v) Loss of Ure2p residues participating in either dimer or prion formation diminishes its ability to carry out NCR-sensitive regulation of Gln3p activity.
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