ArticleThe EMBO journal2001
Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Transposon targeting non-coding RNA transcription targets G/C-rich tracts and is facilitated by an intrinsically disordered protein in Tetrahymena.Science China. Life sciences · 2026Article
- Roles of Noncoding RNAs in Ciliate Genome Architecture.Journal of molecular biology · 2020Review
- Programmed genome rearrangements in Oxytricha produce transcriptionally active extrachromosomal circular DNA.Nucleic acids research · 2019Article
- Diversification of small RNA amplification mechanisms for targeting transposon-related sequences in ciliates.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structure of the germline genome ofeLife · 2016Article
- Ku-mediated coupling of DNA cleavage and repair during programmed genome rearrangements in the ciliate Paramecium tetraurelia.PLoS genetics · 2014Article
- A domesticated PiggyBac transposase interacts with heterochromatin and catalyzes reproducible DNA elimination in Tetrahymena.PLoS genetics · 2013Article
- An essential role for the DNA breakage-repair protein Ku80 in programmed DNA rearrangements in Tetrahymena thermophila.Molecular biology of the cell · 2012Article
- The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.PLoS genetics · 2012Article
- RNA-Mediated Epigenetic Programming of Genome Rearrangements.Annual review of genomics and human genetics · 2011Review
- The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.Eukaryotic cell · 2010Article
- Processing of double-strand breaks is involved in the precise excision of paramecium internal eliminated sequences.Molecular and cellular biology · 2003Article
- PCR-based detection of a rare linear DNA in cell culture.Biological procedures online · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA sequences (IES elements) eliminated from the developing macronucleus in the ciliate Tetrahymena thermophila are released as linear fragments, which have now been detected and isolated. A PCR-mediated examination of fragment end structures reveals three types of strand scission events, reflecting three steps in the deletion process. New evidence is provided for two steps proposed previously: an initiating double-stranded cleavage, and strand transfer to create a branched deletion intermediate. The fragment ends provide evidence for a previously uncharacterized third step: the branched DNA strand is cleaved at one of several defined sites located within 15-16 nucleotides of the IES boundary, liberating the deleted DNA in a linear form.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.