ArticleThe EMBO journal2001
Specific targeting of insect and vertebrate telomeres with pyrrole and imidazole polyamides.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 82 citations in OpenAlex.
- Mitochondria: Endosymbiont bacteria DNA sequence as a target against cancer.Cancer science · 2021Review
- Telomere-specific chromatin capture using a pyrrole-imidazole polyamide probe for the identification of proteins and non-coding RNAs.Epigenetics & chromatin · 2021Article
- A Near-Infrared Fluorogenic Pyrrole-Imidazole Polyamide Probe for Live-Cell Imaging of Telomeres.Journal of the American Chemical Society · 2020Article
- Submolecular dissection reveals strong and specific binding of polyamide-pyridostatin conjugates to human telomere interface.Nucleic acids research · 2019Article
- Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production.Scientific reports · 2017Article
- Article
- Telomere Visualization in Tissue Sections using Pyrrole-Imidazole Polyamide Probes.Scientific reports · 2016Article
- A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma.Quarterly reviews of biophysics · 2015Review
- Tandem trimer pyrrole-imidazole polyamide probes targeting 18 base pairs in human telomere sequences.Chemical science · 2015Article
- DNA Binding Polyamides and the Importance of DNA Recognition in their use as Gene-Specific and Antiviral Agents.Medicinal chemistry · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Programmable DNA-binding small molecules.Bioorganic & medicinal chemistry · 2013Review
- Article
- Completion of a Programmable DNA-Binding Small Molecule Library.Tetrahedron · 2007Article
- Targeting human telomerase for cancer therapeutics.Cytotechnology · 2004Article
- Influence of structural variation on nuclear localization of DNA-binding polyamide-fluorophore conjugates.Nucleic acids research · 2004Article
- Visualizing telomere dynamics in living mammalian cells using PNA probes.The EMBO journal · 2003Article
- Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level.Nucleic acids research · 2003Article
- Inhibition of human papilloma virus E2 DNA binding protein by covalently linked polyamides.Nucleic acids research · 2003Article
- Use of fluorescent sequence-specific polyamides to discriminate human chromosomes by microscopy and flow cytometry.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA minor groove-binding compounds (polyamides) that target insect and vertebrate telomeric repeats with high specificity were synthesized. Base pair recognition of these polyamides is based on the presence of the heterocyclic amino acids pyrrole and imidazole. One compound (TH52B) interacts uniquely and with excellent specificity (K(d) = 0.12 nM) with two consecutive insect-type telomeric repeats (TTAGG). A related compound, TH59, displays high specificity (K(d) = 0.5 nM) for tandem vertebrate (TTAGGG) and insect telomeric repeats. The high affinity and specificity of these compounds were achieved by bidentate binding of two flexibly linked DNA-binding moieties. Epifluorescence microscopy studies show that fluorescent derivatives of TH52B and TH59 stain insect or vertebrate telomeres of chromosomes and nuclei sharply. Importantly, the telomere-specific polyamide signals of HeLa chromosomes co-localize with the immunofluorescence signals of the telomere-binding protein TRF1. Our results demonstrate that telomere-specific compounds allow rapid estimation of relative telomere length. The insect-specific compound TH52 was shown to be incorporated rapidly into growing Sf9 cells, underlining the potential of these compounds for telomere biology and possibly human medicine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.