ArticleThe EMBO journal2001
Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 89 citations in OpenAlex.
- Atg18 interaction positions Atg2 for efficient lipid transfer into phagophore elongation.The EMBO journal · 2026Article
- Pex3 promotes formation of peroxisome-peroxisome and peroxisome-lipid droplet contact sites.Scientific reports · 2025Article
- A plasmid module for PCR-based gene modification for the accurate measurement of vacuolar delivery of specific proteins in yeastAutophagy reports · 2025Article
- Analysis of Protein Cysteine Acylation Using a Modified Suspension Trap (Acyl-Trap).Journal of proteome research · 2024Article
- Analysis of Protein Cysteine Acylation Using a Modified Suspension Trap (Acyl-Trap).bioRxiv : the preprint server for biology · 2024Article
- The Atg1 complex, Atg9, and Vac8 recruit PI3K complex I to the pre-autophagosomal structure.The Journal of cell biology · 2023Article
- Structures of Vac8-containing protein complexes reveal the underlying mechanism by which Vac8 regulates multiple cellular processes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Spatial control of avidity regulates initiation and progression of selective autophagy.Nature communications · 2021Article
- Vac8 spatially confines autophagosome formation at the vacuole inJournal of cell science · 2019Article
- Article
- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts.The Journal of cell biology · 2015Article
- Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae.The Journal of biological chemistry · 2014Article
- Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Molecular and cellular biology · 2013Article
- Article
- Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3.The Journal of biological chemistry · 2009Article
- Vacuole inheritance regulates cell size and branching frequency of Candida albicans hyphae.Molecular microbiology · 2009Article
- Use of micellar electrokinetic chromatography to measure palmitoylation of a peptide.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2008Article
- Rho Family GTPase modification and dependence on CAAX motif-signaled posttranslational modification.The Journal of biological chemistry · 2008Article
- Role of the V-ATPase in regulation of the vacuolar fission-fusion equilibrium.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activated fatty acids stimulate budding and fusion in several cell-free assays for vesicular transport. This stimulation is thought to be due to protein palmitoylation, but relevant substrates have not yet been identified. We now report that Vac8p, a protein known to be required for vacuole inheritance, becomes palmitoylated when isolated yeast vacuoles are incubated under conditions that allow membrane fusion. Similar requirements for Vac8p palmitoylation and vacuole fusion, the inhibition of vacuole fusion by antibodies to Vac8p and the strongly reduced fusion of vacuoles lacking Vac8p suggest that palmitoylated Vac8p is essential for homotypic vacuole fusion. Strikingly, palmitoylation of Vac8p is blocked by the addition of antibodies to Sec18p (yeast NSF) only. Consistent with this, a portion of Vac8p is associated with the SNARE complex on vacuoles, which is lost during Sec18p- and ATP-dependent priming. During or after SNARE complex disassembly, palmitoylation occurs and anchors Vac8p to the vacuolar membrane. We propose that palmitoylation of Vac8p is regulated by the same machinery that controls membrane fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.