ArticleGenetics2001
The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 37 citations in OpenAlex.
- From in vitro to in vivo reprogramming for neural transdifferentiation: An approach for CNS tissue remodeling using stem cell technology.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Review
- The 3' to 5' Exoribonuclease DIS3: From Structure and Mechanisms to Biological Functions and Role in Human Disease.Biomolecules · 2015Review
- Rrp12 and the Exportin Crm1 participate in late assembly events in the nucleolus during 40S ribosomal subunit biogenesis.PLoS genetics · 2014Article
- Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility.Nature genetics · 2012Article
- Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.Yeast (Chichester, England) · 2011Article
- The exozyme model: a continuum of functionally distinct complexes.RNA (New York, N.Y.) · 2011Review
- Drosophila melanogaster Dis3 N-terminal domains are required for ribonuclease activities, nuclear localization and exosome interactions.Nucleic acids research · 2010Article
- Structural and biochemical characterization of CRN-5 and Rrp46: an exosome component participating in apoptotic DNA degradation.RNA (New York, N.Y.) · 2010Article
- Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast.Molecular and cellular biology · 2010Article
- The final step in 5.8S rRNA processing is cytoplasmic in Saccharomyces cerevisiae.Molecular and cellular biology · 2010Article
- Interdependent nucleocytoplasmic trafficking and interactions of Dis3 with Rrp6, the core exosome and importin-alpha3.Traffic (Copenhagen, Denmark) · 2009Article
- SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport.PloS one · 2008Article
- An inventory of yeast proteins associated with nucleolar and ribosomal components.Genome biology · 2006Article
- NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.Nucleic acids research · 2006Article
- Functional link between ribosome formation and biogenesis of iron-sulfur proteins.The EMBO journal · 2005Article
- A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits.Genes & development · 2004Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dis3p, a subunit of the exosome, interacts directly with Ran. To clarify the relationship between the exosome and the RanGTPase cycle, a series of temperature-sensitive Saccharomyces cerevisiae dis3 mutants were isolated and their 5.8S rRNA processing was compared with processing in strains with mutations in a S. cerevisiae Ran homologue, Gsp1p. In both dis3 and gsp1 mutants, 3' processing of 7S-to-5.8S rRNA was blocked at three identical sites in an allele-specific manner. In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3. Inhibition of 5.8S rRNA maturation in gsp1 was rescued by overexpression of nuclear exosome components Dis3p, Rrp4p, and Mtr4p, but not by a cytoplasmic exosome component, Ski2p. Furthermore, gsp1 and dis3 accumulated the 5'-A0 fragment of 35S pre-rRNA, which is also degraded by the exosome, and the level of 27S rRNA was reduced. Neither 5.8S rRNA intermediates nor 5'-A0 fragments were observed in mutants defective in the nucleocytoplasmic transport, indicating that Gsp1p regulates rRNA processing through Dis3p, independent of nucleocytoplasmic transport.
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