ArticleGenetics2001
Yeast spt6-140 mutation, affecting chromatin and transcription, preferentially increases recombination in which Rad51p-mediated strand exchange is dispensable.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Insights into Spt6: a histone chaperone that functions in transcription, DNA replication, and genome stability.Trends in genetics : TIG · 2023Review
- The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability.Cell reports · 2023Article
- Histone depletion prevents telomere fusions in pre-senescent cells.PLoS genetics · 2018Article
- Caffeine inhibits gene conversion by displacing Rad51 from ssDNA.Nucleic acids research · 2015Article
- Histone deacetylases and phosphorylated polymerase II C-terminal domain recruit Spt6 for cotranscriptional histone reassembly.Molecular and cellular biology · 2014Article
- A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation.Nucleic acids research · 2014Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Differential genetic interactions between Sgs1, DNA-damage checkpoint components and DNA repair factors in the maintenance of chromosome stability.Genome integrity · 2011Article
- Spt6 is required for heterochromatic silencing in the fission yeast Schizosaccharomyces pombe.Molecular and cellular biology · 2011Article
- Article
- Chromosomal translocations caused by either pol32-dependent or pol32-independent triparental break-induced replication.Molecular and cellular biology · 2009Article
- Article
- Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication.Nucleic acids research · 2007Article
- Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- Partial depletion of histone H4 increases homologous recombination-mediated genetic instability.Molecular and cellular biology · 2005Article
- Article
- The absence of the yeast chromatin assembly factor Asf1 increases genomic instability and sister chromatid exchange.EMBO reports · 2004Article
- Genome instability in rad54 mutants of Saccharomyces cerevisiae.Nucleic acids research · 2003Article
- The roles of REV3 and RAD57 in double-strand-break-repair-induced mutagenesis of Saccharomyces cerevisiae.Genetics · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown that the spt6-140 and spt4-3 mutations, affecting chromatin structure and transcription, stimulate recombination between inverted repeats by a RAD52-dependent mechanism that is very efficient in the absence of RAD51, RAD54, RAD55, and RAD57. Such a mechanism of recombination is RAD1-RAD59-dependent and yields gene conversions highly associated with the inversion of the repeat. The spt6-140 mutation alters transcription and chromatin in our inverted repeats, as determined by Northern and micrococcal nuclease sensitivity analyses, respectively. Hyper-recombination levels are diminished in the absence of transcription. We believe that the chromatin alteration, together with transcription impairment caused by spt6-140, increases the incidence of spontaneous recombination regardless of whether or not it is mediated by Rad51p-dependent strand exchange. Our results suggest that spt6, as well as spt4, primarily stimulates a mechanism of break-induced replication. We discuss the possibility that the chromatin alteration caused by spt6-140 facilitates a Rad52p-mediated one-ended strand invasion event, possibly inefficient in wild-type chromatin. Our results are consistent with the idea that the major mechanism leading to inversions might not be crossing over but break-induced replication followed by single-strand annealing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.