ArticleOncogene2001
Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by IkappaBbeta.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Development of human alveolar epithelial cell models to study distal lung biology and disease.iScience · 2022Article
- Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer.Cancer cell · 2021Article
- BRMS1: a multifunctional signaling molecule in metastasis.Cancer metastasis reviews · 2020Review
- The role of Rac1 in the regulation of NF-κB activity, cell proliferation, and cell migration in non-small cell lung carcinoma.Cancer biology & therapy · 2012Article
- Anti-cancer activity of a novel small molecule compound that simultaneously activates p53 and inhibits NF-κB signaling.PloS one · 2012Article
- Non-small cell lung carcinoma cell motility, rac activation and metastatic dissemination are mediated by protein kinase C epsilon.PloS one · 2012Article
- NF-kappaB in lung tumorigenesis.Cancers · 2011Article
- Gankyrin plays an essential role in Ras-induced tumorigenesis through regulation of the RhoA/ROCK pathway in mammalian cells.The Journal of clinical investigation · 2010Article
- Subcellular localization of apurinic endonuclease 1 promotes lung tumor aggressiveness via NF-kappaB activation.Oncogene · 2010Article
- Pharmacokinetic properties and antitumor efficacy of the 5-fluorouracil loaded PEG-hydrogel.BMC cancer · 2010Article
- Use of bioluminescent imaging to investigate the role of nuclear factor-kappaBeta in experimental non-small cell lung cancer metastasis.Clinical & experimental metastasis · 2008Article
- NF-kappaB-independent sensitization of glioblastoma cells for TRAIL-induced apoptosis by proteasome inhibition.Oncogene · 2007Article
- Nuclear factor-kappaB and inhibitor of kappaB kinase pathways in oncogenic initiation and progression.Oncogene · 2006Review
- Tumor metastasis: mechanistic insights and clinical challenges.Nature medicine · 2006Review
- Article
- Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies.BMC cancer · 2005Article
- Identification of a human NF-kappaB-activating protein, TAB3.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- pRB2/p130 target genes in non-small lung cancer cells identified by microarray analysis.Oncogene · 2003Article
- Genetic alterations of multiple tumor suppressors and oncogenes in the carcinogenesis and progression of lung cancer.Oncogene · 2002Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
To evaluate the role of the NF-kappaB signaling pathway in oncogenic transformation, we expressed IkappaBbeta, a specific inhibitor of NF-kappaB, in two human lung adenocarcinoma cell lines, A549 and H441. Expression of IkappaBbeta significantly reduced NF-kappaB activation induced by cotransfection with p65/RelA or TNF-alpha and abrogated the basal NF-kappaB activity in A549 cells. Transfection of IkappaBbeta into A549, H441 and K-ras-transformed NIH3T3 cells suppressed anchorage-independent growth as measured by colony formation in soft agar. Anchorage-independent growth of vector-transfected A549 cells in reduced serum could be enhanced by both EGF and IGF-I. In contrast, only EGF but not IGF-I could induce anchorage-independent growth of IkappaBbeta-expressing A549 cells, suggesting that the IGF-I signaling pathway regulating growth and survival may be blocked by IkappaBbeta. Interestingly, expression of IkappaBbeta suppressed growth of A549 cells in low serum in vitro without affecting in vivo growth subcutaneously in nude mice. However, metastatic growth of IkappaBbeta-expressing A549 cells in the lungs of nude mice was significantly inhibited. These results provide evidence that NFkappaB plays an important role in anchorage-independent growth and metastatic growth of lung carcinoma cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.