ArticlePlant physiology2001
Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 208 papers.
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208 citing papers in PubMed, 540 citations in OpenAlex.
- Opposite diacylglycerol enantiomeric specificities of Arabidopsis DGAT1 and DGAT2 reveal distinct roles in TAG synthesis.Plant physiology · 2026Article
- Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026Review
- Overexpression of Chlorella ellipsoidea DGAT1 gene in soybean improves oil content and influences lipid metabolism.Planta · 2026Article
- A single-MYB transcription factor GmMYB331 regulates seed oil accumulation and seed size/weight in soybean.Journal of integrative plant biology · 2026Article
- Lipid regulation inFrontiers in plant science · 2026Review
- Functional diversity of sunflower DGAT1 and DGAT2 enzymes underlying seed triacylglycerol biosynthesis.Frontiers in plant science · 2026Article
- Quantitative proteomic analysis of Arabidopsis thaliana with different levels of phospholipid:diacylglycerol acyltransferase1 expression.BMC genomics · 2025Article
- Discovery of Hub Genes Involved in Seed Development and Lipid Biosynthesis in Sea Buckthorn (Plants (Basel, Switzerland) · 2025Article
- Transcriptome Analysis Reveals Metabolic Pathways and Key Genes Involved in Oleic Acid Formation of Sunflower (International journal of molecular sciences · 2025Article
- Effects of DpWRI1-like gene overexpression in transgenic Dunaliella parva.Antonie van Leeuwenhoek · 2025Article
- Seed-specific expression of phosphatidate phosphohydrolases increases soybean oil content and seed weight.Biotechnology for biofuels and bioproducts · 2025Article
- BnLPAT2 gene regulates oil accumulation in Brassica napus by modulating linoleic and linolenic acid levels in seeds.PloS one · 2025Article
- Regulatory mechanism of carbohydrate metabolism pathways on oil biosynthesis of oil plantFrontiers in plant science · 2025Article
- The first intron and promoter of Arabidopsis DIACYLGLYCEROL ACYLTRANSFERASE 1 exert synergistic effects on pollen and embryo lipid accumulation.The New phytologist · 2025Article
- Metabolic engineering-induced transcriptome reprogramming of lipid biosynthesis enhances oil composition in oat.Plant biotechnology journal · 2024Article
- De novo assembly ofHeliyon · 2024Article
- Regulation of Oil Biosynthesis and Genetic Improvement in Plants: Advances and Prospects.Genes · 2024Review
- A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content.Plant & cell physiology · 2024Article
- Functional Characterization of the Effects of CsDGAT1 and CsDGAT2 on Fatty Acid Composition inInternational journal of molecular sciences · 2024Article
- Comparative transcriptomic analysis provides insights into the genetic networks regulating oil differential production in oil crops.BMC biology · 2024Article
148 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently reported the cloning and characterization of an Arabidopsis (ecotype Columbia) diacylglycerol acyltransferase cDNA (Zou et al., 1999) and found that in Arabidopsis mutant line AS11, an ethyl methanesulfonate-induced mutation at a locus on chromosome II designated as Tag1 consists of a 147-bp insertion in the DNA, which results in a repeat of the 81-bp exon 2 in the Tag1 cDNA. This insertion mutation is correlated with an altered seed fatty acid composition, reduced diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) activity, reduced seed triacylglycerol content, and delayed seed development in the AS11 mutant. The effect of the insertion mutation on microsomal acyl-coenzyme A-dependent DGAT is examined with respect to DGAT activity and its substrate specificity in the AS11 mutant relative to wild type. We demonstrate that transformation of mutant AS11 with a single copy of the wild-type Tag1 DGAT cDNA can complement the fatty acid and reduced oil phenotype of mutant AS11. More importantly, we show for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels. The DGAT activity in developing seed of transgenic lines was enhanced by 10% to 70%. Thus, the current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds.
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