ArticlePlant physiology2001
Proteomic analysis of arabidopsis seed germination and priming.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
What it found
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Who cites it
144 citing papers in PubMed, 627 citations in OpenAlex.
- Translational and epitranscriptomic regulation of seed germination in Arabidopsis thaliana genotypes with contrasting dormancy phenotypes.Plant molecular biology · 2025Article
- Evolutionary and functional relationships between plant and microbial CThe New phytologist · 2025Review
- Modifications in Carbon and Nitrogen Metabolites ofPlants (Basel, Switzerland) · 2025Article
- Regulation of seed germination: ROS, epigenetic, and hormonal aspects.Journal of advanced research · 2025Review
- Melatonin Enhances Maize Germination, Growth, and Salt Tolerance by Regulating Reactive Oxygen Species Accumulation and Antioxidant Systems.Plants (Basel, Switzerland) · 2025Article
- Unravelling the Significance of Seed Proteomics: Insights into Seed Development, Function, and Agricultural Applications.The protein journal · 2024Review
- Deciphering physiological and transcriptional mechanisms of maize seed germination.Plant molecular biology · 2024Article
- An ecotype-specific effect of osmopriming and melatonin during salt stress in Arabidopsis thaliana.BMC plant biology · 2024Article
- Application of Methionine Increases the Germination Rate of Maize Seeds by Triggering Multiple Phenylpropanoid Biosynthetic Genes at Transcript Levels.Plants (Basel, Switzerland) · 2023Article
- When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates.Chemical reviews · 2023Review
- Comparative Effects of Hydropriming and Iron Priming on Germination and Seedling Morphophysiological Attributes of Stay-Green Wheat.ACS omega · 2023Article
- Food Allergens of Plant Origin.Foods (Basel, Switzerland) · 2023Review
- Isopropylmalate synthasePlant biotechnology (Tokyo, Japan) · 2023Article
- Responses of the tree peony (Paeonia suffruticosa, Paeoniaceae) cultivar 'Yu Hong' to heat stress revealed by iTRAQ-based quantitative proteomics.Proteome science · 2022Article
- Zn application through seed priming improves productivity and grain nutritional quality of silage corn.Saudi journal of biological sciences · 2022Article
- Combined transcriptomic and metabolomic analysis reveals the potential mechanism of seed germination and young seedling growth in Tamarix hispida.BMC genomics · 2022Article
- Methods to promote seed germination in the lacquer tree, Toxicodendron vernicifluum (Stokes) F.A. Barkley.PloS one · 2022Article
- Article
- Carrot (Frontiers in plant science · 2022Article
- Live imagingSTAR protocols · 2021Article
84 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better understand seed germination, a complex developmental process, we developed a proteome analysis of the model plant Arabidopsis for which complete genome sequence is now available. Among about 1,300 total seed proteins resolved in two-dimensional gels, changes in the abundance (up- and down-regulation) of 74 proteins were observed during germination sensu stricto (i.e. prior to radicle emergence) and the radicle protrusion step. This approach was also used to analyze protein changes occurring during industrial seed pretreatments such as priming that accelerate seed germination and improve seedling uniformity. Several proteins were identified by matrix-assisted laser-desorption ionization time of flight mass spectrometry. Some of them had previously been shown to play a role during germination and/or priming in several plant species, a finding that underlines the usefulness of using Arabidopsis as a model system for molecular analysis of seed quality. Furthermore, the present study, carried out at the protein level, validates previous results obtained at the level of gene expression (e.g. from quantitation of differentially expressed mRNAs or analyses of promoter/reporter constructs). Finally, this approach revealed new proteins associated with the different phases of seed germination and priming. Some of them are involved either in the imbibition process of the seeds (such as an actin isoform or a WD-40 repeat protein) or in the seed dehydration process (e.g. cytosolic glyceraldehyde-3-phosphate dehydrogenase). These facts highlight the power of proteomics to unravel specific features of complex developmental processes such as germination and to detect protein markers that can be used to characterize seed vigor of commercial seed lots and to develop and monitor priming treatments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.