ArticleThe Journal of cell biology2001
Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 186 citations in OpenAlex.
- Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025Article
- Mouse radial spoke 3 is a metabolic and regulatory hub in cilia.Nature structural & molecular biology · 2025Article
- Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development.Nature communications · 2025Article
- Structure and molecular basis of spermatid elongation in theOpen biology · 2023Review
- Radial spoke protein 9 is necessary for axoneme assembly in Plasmodium but not in trypanosomatid parasites.Journal of cell science · 2023Article
- LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice.Journal of cell science · 2021Article
- Heme-binding protein CYB5D1 is a radial spoke component required for coordinated ciliary beating.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Composition and function of ciliary inner-dynein-arm subunits studied in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Structures of radial spokes and associated complexes important for ciliary motility.Nature structural & molecular biology · 2021Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- Review
- Proteome of the central apparatus of a ciliary axoneme.The Journal of cell biology · 2019Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- Biallelic mutations of CFAP251 cause sperm flagellar defects and human male infertility.Journal of human genetics · 2019Article
- The flagellar protein Enkurin is required for mouse sperm motility and for transport through the female reproductive tract.Biology of reproduction · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Physiology and Evolution of Voltage-Gated Calcium Channels in Early Diverging Animal Phyla: Cnidaria, Placozoa, Porifera and Ctenophora.Frontiers in physiology · 2016Article
- The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia.Molecular biology of the cell · 2015Article
- Detailed structural and biochemical characterization of the nexin-dynein regulatory complex.Molecular biology of the cell · 2015Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic and in vitro analyses have revealed that radial spokes play a crucial role in regulation of ciliary and flagellar motility, including control of waveform. However, the mechanisms of regulation are not understood. Here, we developed a novel procedure to isolate intact radial spokes as a step toward understanding the mechanism by which these complexes regulate dynein activity. The isolated radial spokes sediment as 20S complexes that are the size and shape of radial spokes. Extracted radial spokes rescue radial spoke structure when reconstituted with isolated axonemes derived from the radial spoke mutant pf14. Isolated radial spokes are composed of the 17 previously defined spoke proteins as well as at least five additional proteins including calmodulin and the ubiquitous dynein light chain LC8. Analyses of flagellar mutants and chemical cross-linking studies demonstrated calmodulin and LC8 form a complex located in the radial spoke stalk. We postulate that calmodulin, located in the radial spoke stalk, plays a role in calcium control of flagellar bending.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.