ArticleInfection and immunity2001
In vivo visualization of bacterial colonization, antigen expression, and specific T-cell induction following oral administration of live recombinant Salmonella enterica serovar Typhimurium.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Antigen-specific activation of gut immune cells drives autoimmune neuroinflammation.Gut microbes · 2026Article
- Metabolic profiling and genetic tool development in the mucosal bacterium Selenomonas sputigena.Genes & genomics · 2025Article
- Efficient fluorescence-based localization technique for real-time tracking endophytes route in host-plants colonization.Plant direct · 2022Article
- Optimal protection againstProceedings of the National Academy of Sciences of the United States of America · 2018Article
- DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli.BMC microbiology · 2017Article
- Imaging flow cytometry analysis of intracellular pathogens.Methods (San Diego, Calif.) · 2017Review
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- Immunity to intestinal pathogens: lessons learned from Salmonella.Immunological reviews · 2014Review
- Article
- Immunity to intracellular Salmonella depends on surface-associated antigens.PLoS pathogens · 2012Article
- Development of protective immunity to Salmonella, a mucosal pathogen with a systemic agenda.Mucosal immunology · 2011Review
- Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.Applied and environmental microbiology · 2010Article
- Vaccine-induced antibody isotypes are skewed by impaired CD4 T cell and invariant NKT cell effector responses in MyD88-deficient mice.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Tracking the dynamics of salmonella specific T cell responses.Current topics in microbiology and immunology · 2009Review
- Gamma interferon-independent effects of interleukin-12 on immunity to Salmonella enterica serovar Typhimurium.Infection and immunity · 2007Article
- Cloning vectors and fluorescent proteins can significantly inhibit Salmonella enterica virulence in both epithelial cells and macrophages: implications for bacterial pathogenesis studies.Infection and immunity · 2005Article
- Review
- Enhanced immunogenicity in the murine airway mucosa with an attenuated Salmonella live vaccine expressing OprF-OprI from Pseudomonas aeruginosa.Infection and immunity · 2004Article
- Antigen selection based on expression levels during infection facilitates vaccine development for an intracellular pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Role of neutrophils in murine salmonellosis.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live attenuated Salmonella strains that express a foreign antigen are promising oral vaccine candidates. Numerous genetic modifications have been empirically tested, but their effects on immunogenicity are difficult to interpret since important in vivo properties of recombinant Salmonella strains such as antigen expression and localization are incompletely characterized and the crucial early inductive events of an immune response to the foreign antigen are not fully understood. Here, methods were developed to directly localize and quantitate the in situ expression of an ovalbumin model antigen in recombinant Salmonella enterica serovar Typhimurium using two-color flow cytometry and confocal microscopy. In parallel, the in vivo activation, blast formation, and division of ovalbumin-specific CD4(+) T cells were followed using a well-characterized transgenic T-cell receptor mouse model. This combined approach revealed a biphasic induction of ovalbumin-specific T cells in the Peyer's patches that followed the local ovalbumin expression of orally administered recombinant Salmonella cells in a dose- and time-dependent manner. Interestingly, intact Salmonella cells and cognate T cells seemed to remain in separate tissue compartments throughout induction, suggesting a transport of killed Salmonella cells from the colonized subepithelial dome area to the interfollicular inductive sites. The findings of this study will help to rationally optimize recombinant Salmonella strains as efficacious live antigen carriers for oral vaccination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.