ArticleInfection and immunity2001
Gram-positive and gram-negative bacteria do not trigger monocytic cytokine production through similar intracellular pathways.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Commensal Peptoniphilus harei induce activation of monocytes via TLR2/CD14 signalling in whole blood.Medical microbiology and immunology · 2025Article
- Plasma microRNAs as non-invasive biomarkers in bovine endometritis caused by Gram-negative and Gram-positive bacteria.Veterinary research forum : an international quarterly journal · 2023Article
- Antibiotics as immunomodulators: a potential pharmacologic approach for ARDS treatment.European respiratory review : an official journal of the European Respiratory Society · 2021Review
- Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy.International journal of molecular sciences · 2021Article
- Differential engulfment ofEXCLI journal · 2020Article
- Enhanced survival of Leishmania major in neutrophil granulocytes in the presence of apoptotic cells.PloS one · 2017Article
- The expression of inflammatory cytokines, TAM tyrosine kinase receptors and their ligands is upregulated in venous leg ulcer patients: a novel insight into chronic wound immunity.International wound journal · 2016Article
- Vitamin D regulates contractile profile in human uterine myometrial cells via NF-κB pathway.American journal of obstetrics and gynecology · 2014Article
- Divergent responses to peptidoglycans derived from different E. coli serotypes influence inflammatory outcome in trout, Oncorhynchus mykiss, macrophages.BMC genomics · 2011Article
- Toll-like receptor-mediated production of IL-1Ra is negatively regulated by GSK3 via the MAPK ERK1/2.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Response of monocytes exposed to phagocytosable particles and discs of comparable surface roughness.Biomaterials · 2007Article
- Circulating inflammatory mediators during start of fever in differential diagnosis of gram-negative and gram-positive infections in leukopenic rats.Clinical and diagnostic laboratory immunology · 2005Article
- Recognition of Staphylococcus aureus by the innate immune system.Clinical microbiology reviews · 2005Review
- The immunomodulating neuropeptide alpha-melanocyte-stimulating hormone (alpha-MSH) suppresses LPS-stimulated TLR4 with IRAK-M in macrophages.Journal of neuroimmunology · 2005Article
- Recombinant soluble CD14 reduces severity of intramammary infection by Escherichia coli.Infection and immunity · 2003Article
- Role of innate immune factors in the adjuvant activity of monophosphoryl lipid A.Infection and immunity · 2003Article
- Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.World journal of gastroenterology · 2002Article
- Binding of LFA-1 (CD11a) to intercellular adhesion molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) triggers transmigration of human immunodeficiency virus type 1-infected monocytes through mucosal epithelial cells.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toll-like receptors (TLRs) are involved in human monocyte activation by lipopolysaccharide (LPS) and Staphylococcus aureus Cowan (SAC), suggesting that gram-positive and gram-negative bacteria may trigger similar intracellular events. Treatment with specific kinase inhibitors prior to cell stimulation dramatically decreased LPS-induced cytokine production. Blocking of the p38 pathway prior to LPS stimulation decreased interleukin-1alpha (IL-1alpha), IL-1ra, and tumor necrosis factor alpha (TNF-alpha) production, whereas blocking of the ERK1/2 pathways inhibited IL-1alpha, IL-1beta, and IL-1ra but not TNF-alpha production. When cells were stimulated by SAC, inhibition of the p38 pathway did not affect cytokine production, whereas only IL-1alpha production was decreased in the presence of ERK kinase inhibitor. We also demonstrated that although LPS and SAC have been shown to bind to CD14 before transmitting signals to TLR4 and TLR2, respectively, internalization of CD14 occurred only in monocytes triggered by LPS. Pretreatment of the cells with SB203580, U0126, or a mixture of both inhibitors did not affect internalization of CD14. Altogether, these results suggest that TLR2 signaling does not involve p38 mitogen-activated protein kinase signaling pathways, indicating that divergent pathways are triggered by gram-positive and gram-negative bacteria, thereby inducing cytokine production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.