ArticleInfection and immunity2001
Deletion of wboA enhances activation of the lectin pathway of complement in Brucella abortus and Brucella melitensis.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- The mechanism of chronic intracellular infection withFrontiers in cellular and infection microbiology · 2023Review
- Review
- Immunosuppressive Mechanisms in Brucellosis in Light of Chronic Bacterial Diseases.Microorganisms · 2022Review
- Adhesins ofPathogens (Basel, Switzerland) · 2020Review
- The Role ofPathogens (Basel, Switzerland) · 2019Review
- Lethality ofVirulence · 2019Article
- Depletion of Complement Enhances the Clearance of Brucella abortus in Mice.Infection and immunity · 2018Article
- N-Formyl-Perosamine Surface Homopolysaccharides Hinder the Recognition of Brucella abortus by Mouse Neutrophils.Infection and immunity · 2016Article
- Smooth to Rough Dissociation in Brucella: The Missing Link to Virulence.Frontiers in cellular and infection microbiology · 2015Article
- Single-molecule interrogation of a bacterial sugar transporter allows the discovery of an extracellular inhibitor.Nature chemistry · 2013Article
- Brucella melitensis MucR, an orthologue of Sinorhizobium meliloti MucR, is involved in resistance to oxidative, detergent, and saline stresses and cell envelope modifications.Journal of bacteriology · 2013Article
- The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence.PloS one · 2013Article
- Article
- Article
- Lipopolysaccharide-Deficient Brucella Variants Arise Spontaneously during Infection.Frontiers in microbiology · 2011Article
- Article
- Chronic brucellosis patients retain low frequency of CD4+ T-lymphocytes expressing CD25 and CD28 after Escherichia coli LPS stimulation of PHA-cultured PBMCs.Clinical & developmental immunology · 2008Article
- Interaction of Brucella suis and Brucella abortus rough strains with human dendritic cells.Infection and immunity · 2007Article
- Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis.Infection and immunity · 2007Article
- CD80/CD28 co-stimulation in human brucellosis.Clinical and experimental immunology · 2006Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on their surface. These OPS help the bacteria avoid the bactericidal action of serum. The wboA gene, coding for the enzyme glycosyltransferase, is essential for the synthesis of O chain in Brucella. In this study, the sensitivity to serum of smooth, virulent Brucella melitensis 16M and B. abortus 2308, rough wboA mutants VTRM1, RA1, and WRR51 derived from these two Brucella species, and the B. abortus vaccine strain RB51 was assayed using normal nonimmune human serum (NHS). The deposition of complement components and mannose-binding lectin (MBL) on the bacterial surface was detected by flow cytometry. Rough B. abortus mutants were more sensitive to the bactericidal action of NHS than were rough B. melitensis mutants. Complement components were deposited on smooth strains at a slower rate compared to rough strains. Deposition of iC3b and C5b-9 and bacterial killing occurred when bacteria were treated with C1q-depleted, but not with C2-depleted serum or NHS in the presence of Mg-EGTA. These results indicate that (i) OPS-deficient strains derived from B. melitensis 16M are more resistant to the bactericidal action of NHS than OPS-deficient strains derived from B. abortus 2308, (ii) both the classical and the MBL-mediated pathways are involved in complement deposition and complement-mediated killing of Brucella, and (iii) the alternative pathway is not activated by smooth or rough brucellae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.