ArticleInfection and immunity2001
Calprotectin expression in vitro by oral epithelial cells confers resistance to infection by Porphyromonas gingivalis.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 137 citations in OpenAlex.
- Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition.FEBS open bio · 2026Article
- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Intracellular calprotectin (S100A8/A9) facilitates DNA damage responses and promotes apoptosis in head and neck squamous cell carcinoma.Oral oncology · 2023Article
- Oral Cavity Calprotectin and Lactoferrin Levels in Relation to Radiotherapy.Current issues in molecular biology · 2022Article
- Calprotectin in nasal secretion: a new biomarker of non-type 2 inflammation in CRSwNP.Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale · 2022Article
- Antimicrobial peptides: Defending the mucosal epithelial barrier.Frontiers in oral health · 2022Review
- Calprotectin (S100A8/A9) Is an Innate Immune Effector in Experimental Periodontitis.Infection and immunity · 2021Article
- [Expression and distribution of calprotectin in healthy and inflamed periodontal tissues].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2021Article
- Salivary Stress/Immunological Markers in Crohn's Disease and Ulcerative Colitis.International journal of molecular sciences · 2020Review
- Intracellular calprotectin (S100A8/A9) controls epithelial differentiation and caspase-mediated cleavage of EGFR in head and neck squamous cell carcinoma.Oral oncology · 2019Article
- Role of Calprotectin as a Biomarker in Periodontal Disease.Mediators of inflammation · 2019Review
- Antimicrobial action of calprotectin that does not involve metal withholding.Metallomics : integrated biometal science · 2018Article
- S100 proteins in rheumatic diseases.Nature reviews. Rheumatology · 2018Review
- Calprotectin and the Initiation and Progression of Head and Neck Cancer.Journal of dental research · 2018Review
- Aberrant MRP14 expression in thyroid follicular cells mediates chemokine secretion through the IL-1β/MAPK pathway in Hashimoto's thyroiditis.Endocrine connections · 2018Article
- An early report: a modified porphyrin-linked metronidazole targeting intracellular Porphyromonas gingivalis in cultured oral epithelial cells.International journal of oral science · 2017Article
- Three/four-dimensional (3D/4D) microscopic imaging and processing in clinical dental research.BMC oral health · 2016Article
- Autonomous immunity in mucosal epithelial cells: fortifying the barrier against infection.Microbes and infection · 2016Review
- Effect of Hangeshashinto on calprotectin expression in human oral epithelial cells.Odontology · 2016Article
- Involvement of calprotectin (S100A8/A9) in molecular pathways associated with HNSCC.Oncotarget · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Calprotectin, an S100 calcium-binding protein with broad-spectrum antimicrobial activity in vitro, is expressed in neutrophils, monocytes, and gingival keratinocytes. In periodontitis, calprotectin appears upregulated and is detected at higher levels in gingival crevicular fluid and tissue specimens. How calprotectin contributes to the pathogenesis of periodontal diseases is unknown. To isolate the effects of calprotectin, a calprotectin-negative oral epithelial cell line was transfected with calprotectin genes to enable expression. Porphyromonas gingivalis was permitted to bind and invade transfected cells expressing calprotectin and sham transfectants. Rates of invasion into both cell lines were compared using the antibiotic protection assay. Transfected cells expressing calprotectin showed 40 to 50% fewer internalized P. gingivalis than sham transfectants. Similarly, binding to calprotectin expressing cells was reduced approximately twofold at all time points (15, 30, 45, and 60 min) as estimated by immunofluorescence analysis. Independent of invasion, however, prolonged exposure to P. gingivalis induced epithelial cell rounding and detachment from the substratum. These morphological changes were delayed, however, in cells expressing calprotectin. Using P. gingivalis protease-deficient mutants, we found that Arg-gingipain and Lys-gingipain contributed to epithelial cell rounding and detachment. In conclusion, expression of calprotectin appears to protect epithelial cells in culture against binding and invasion by P. gingivalis. In addition, cells expressing calprotectin are more resistant to detachment mediated by Arg-gingipain and Lys-gingipain. In periodontal disease, calprotectin may augment both the barrier protection and innate immune functions of the gingival epithelium to promote resistance to P. gingivalis infection.
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