ArticleAmerican journal of physiology. Cell physiology2001
[Ca2+]i as a potential downregulator of alpha2beta1-integrin-mediated A2058 tumor cell migration to type IV collagen.
Article in American journal of physiology. Cell physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Exploring the relationship between total serum calcium and melanoma development: a cross-sectional study.Frontiers in nutrition · 2024Article
- The Role of Calcium Signaling in Melanoma.International journal of molecular sciences · 2022Review
- The Role of Calmodulin in Tumor Cell Migration, Invasiveness, and Metastasis.International journal of molecular sciences · 2020Review
- Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.Acta histochemica et cytochemica · 2015Article
- CYLD inhibits melanoma growth and progression through suppression of the JNK/AP-1 and β1-integrin signaling pathways.The Journal of investigative dermatology · 2013Article
- Transiently entrapped circulating tumor cells interact with neutrophils to facilitate lung metastasis development.Cancer research · 2010Article
- PDGF receptor-{beta} modulates metanephric mesenchyme chemotaxis induced by PDGF AA.American journal of physiology. Renal physiology · 2009Article
- Immunoediting of leukocyte functions within the tumor microenvironment promotes cancer metastasis development.Biorheology · 2009Article
- Melanoma cell extravasation under flow conditions is modulated by leukocytes and endogenously produced interleukin 8.Molecular & cellular biomechanics : MCB · 2005Article
- Distinct role of hydrodynamic shear in leukocyte-facilitated tumor cell extravasation.American journal of physiology. Cell physiology · 2005Article
- Neutrophils influence melanoma adhesion and migration under flow conditions.International journal of cancer · 2003Article
- Article
- In vitro characterization and micromechanics of tumor cell chemotactic protrusion, locomotion, and extravasation.Annals of biomedical engineering · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
We have investigated cellular Ca2+ regulation during A2058 human melanoma cell chemotaxis to type IV collagen (CIV). We have identified alpha2beta1-integrin as the primary mediator of A2058 cell response to CIV in vitro. Integrin ligation initiated a characteristic intracellular Ca2+ concentration ([Ca2+]i) response consisting of an internal release and a receptor-mediated Ca2+ entry. Thapsigargin (TG) pretreatment drained overlapping and CIV-inducible internal Ca2+ stores while initiating a store-operated Ca2+ release (SOCR). CIV-mediated Ca2+ entry was additive to TG-SOCR, suggesting an independent signaling mechanism. Similarly, ionophore application in a basal medium containing Ca2+ initiated a sustained influx. Elevated [Ca2+]i from TG-SOCR or ionophore significantly attenuated cell migration to CIV by recruiting the Ca2+/calcineurin-mediated signaling pathway. Furthermore, low [Ca2+]i induced by EGTA application in the presence of ionophore fully restored cell motility to CIV. Together, these results suggest that [Ca2+]i signaling accompanying A2058 cell response to alpha2beta1-integrin ligation is neither necessary nor sufficient and that elevated [Ca2+]i downregulates cell motility via a calcineurin-mediated mechanism in A2058 cell chemotaxis to CIV.
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