ArticleJournal of bacteriology2001
Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed.
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- Tsrp1 Is a Novel Cyclic Diguanosine Monophosphate Effector that Plays a Role in the Organic-Solvent Tolerance ofJournal of microbiology and biotechnology · 2025Article
- SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.Engineering in life sciences · 2025Article
- Bacterial Tolerance to 1-Butanol and 2-Butanol: Quantitative Assessment and Transcriptomic Response.International journal of molecular sciences · 2024Article
- Adsorption of Flavonoids in a Transcriptional Regulator TtgR: Relative Binding Free Energies and Intermolecular Interactions.The journal of physical chemistry. B · 2024Article
- Assessment of New and Genome-ReducedMicroorganisms · 2023Article
- Insights into the susceptibility of Pseudomonas putida to industrially relevant aromatic hydrocarbons that it can synthesize from sugars.Microbial cell factories · 2023Article
- Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1.BMC microbiology · 2022Article
- Green Extracellular Synthesis of Silver Nanoparticles byMolecules (Basel, Switzerland) · 2022Article
- Gene sharing among plasmids and chromosomes reveals barriers for antibiotic resistance gene transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Recent progress on n-butanol production by lactic acid bacteria.World journal of microbiology & biotechnology · 2021Review
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Adaptive Laboratory Evolution Restores Solvent Tolerance in Plasmid-Cured Pseudomonas putida S12: a Molecular Analysis.Applied and environmental microbiology · 2021Article
- Article
- Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation.Applied microbiology and biotechnology · 2021Article
- Investigation of monoterpenoid resistance mechanisms in Pseudomonas putida and their consequences for biotransformations.Applied microbiology and biotechnology · 2020Article
- Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis.Nature chemical biology · 2020Review
- The effect of organic solvents on selected microorganisms and model liposome membrane.Molecular biology reports · 2019Article
- Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential.World journal of microbiology & biotechnology · 2019Article
- ndpT encodes a new protein involved in nicotine catabolism by Sphingomonas melonis TY.Applied microbiology and biotechnology · 2018Article
33 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
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Abstract
In Pseudomonas putida DOT-T1E multidrug efflux pumps of the resistance-nodulation-division family make a major contribution to solvent resistance. Two pumps have been identified: TtgABC, expressed constitutively, and TtgDEF, induced by aromatic hydrocarbons. A double mutant lacking both efflux pumps was able to survive a sudden toluene shock if and only if preinduced with small amounts of toluene supplied via the gas phase. In this article we report the identification and characterization in this strain of a third efflux pump, named TtgGHI. The ttgGHI genes form an operon that is expressed constitutively at high levels from a single promoter. In the presence of toluene the operon is expressed at an even higher level from two promoters, the constitutive one and a previously unreported one that is inducible and that partially overlaps the constitutive promoter. By site-directed mutagenesis we constructed a single ttgH mutant which was shown to be unable to survive sudden 0.3% (vol/vol) toluene shocks regardless of the preculture conditions. The mutation was transferred to single and double mutants to construct mutant strains in which two or all three pumps are knocked out. Survival analysis of induced and noninduced cells revealed that the TtgABC and TtgGHI pumps extruded toluene, styrene, m-xylene, ethylbenzene, and propylbenzene, whereas the TtgDEF pump removed only toluene and styrene. The triple mutant was hypersensitive to toluene, as shown by its inability to grow with toluene supplied via the vapor phase.
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