ArticleJournal of muscle research and cell motility2000
Hydrogen peroxide modulates Ca2+-activation of single permeabilized fibres from fast- and slow-twitch skeletal muscles of rats.
Article in Journal of muscle research and cell motility, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Increasing indices of frailty in aged female mice are associated with impaired skeletal muscle resilience to downhill running stress.GeroScience · 2026Article
- Respiratory issues in patients with multiple sclerosis as a risk factor during SARS-CoV-2 infection: a potential role for exercise.Molecular and cellular biochemistry · 2023Review
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- Mechanisms Explaining Muscle Fatigue and Muscle Pain in Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): a Review of Recent Findings.Current rheumatology reports · 2017Review
- Exercise-induced oxidative stress: past, present and future.The Journal of physiology · 2016Review
- Contractile properties and sarcoplasmic reticulum calcium content in type I and type II skeletal muscle fibres in active aged humans.The Journal of physiology · 2015Article
- Weakness of whole muscles in mice deficient in Cu, Zn superoxide dismutase is not explained by defects at the level of the contractile apparatus.Age (Dordrecht, Netherlands) · 2013Article
- Accessibility of myofilament cysteines and effects on ATPase depend on the activation state during exposure to oxidants.PloS one · 2013Article
- ROS-mediated decline in maximum Ca2+-activated force in rat skeletal muscle fibers following in vitro and in vivo stimulation.PloS one · 2012Article
- Reactive oxygen species: impact on skeletal muscle.Comprehensive Physiology · 2011Review
- H2O2 alters rat cardiac sarcomere function and protein phosphorylation through redox signaling.American journal of physiology. Heart and circulatory physiology · 2010Article
- Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production.Physiological reviews · 2008Review
- Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres.The Journal of physiology · 2008Article
- Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers.American journal of physiology. Cell physiology · 2008Article
- Age-related decline in actomyosin structure and function.Experimental gerontology · 2007Review
- Effects of oxidation and reduction on contractile function in skeletal muscle fibres of the rat.The Journal of physiology · 2003Article
- Therapeutic clenbuterol treatment does not alter Ca2+ sensitivity of permeabilized fast muscle fibres from exercise trained or untrained horses.Journal of muscle research and cell motility · 2003Article
- Hydrogen peroxide increases depolarization-induced contraction of mechanically skinned slow twitch fibres from rat skeletal muscles.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the effects of redox modulation on single membrane-permeabilized fibre segments from the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus muscles of adult rats to determine whether the contractile apparatus was the redox target responsible for the increased contractility of muscles exposed to low concentrations of H2O2. The effects of H2O2 on maximum Ca2+-activated force were dose-dependent with 30 min exposure to 5 mM H2O2 causing a progressive decrease by 22+/-3 and 13+/-2% in soleus and EDL permeabilized muscle fibres, respectively. Lower concentrations of exogenous H2O2 (100 microM and 1 mM) had no effect on maximum Ca2+-activated force. Subsequent exposure to the reductant dithiothreitol (DTT, 10 mM, 10 min) fully reversed the H2O2-induced depression of force in EDL, but not in soleus muscle fibres. Incubation with DTT alone for 10 min did not alter Ca2+-activated force in either soleus or EDL muscle fibres. The sensitivity of the contractile filaments to Ca2+ (pCa50) was not altered by exposure to any concentration of exogenous H2O2. However, all concentrations of H2O2 diminished the Hill coefficient in permeabilized fibres from the EDL muscle, indicating that the cooperativity of Ca2+ binding to troponin is altered. H2O2 (5 mM) did not affect rigor force, which indicates that the number of crossbridges participating in contraction was not reduced. In conclusion, H2O2 may reduce the maximum Ca2+ activated force production in skinned muscle fibres by decreasing the force per crossbridge.
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