ArticleProceedings of the National Academy of Sciences of the United States of America2001
Thrombin induces the release of the Y-box protein dbpB from mRNA: a mechanism of transcriptional activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition.Scientific reports · 2026Article
- β-Barrels and Amyloids: Structural Transitions, Biological Functions, and Pathogenesis.International journal of molecular sciences · 2021Review
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- Expression and network analysis of YBX1 interactors for identification of new drug targets in lung adenocarcinoma.Journal of genomics · 2018Article
- Activated Protein C Ameliorates Renal Ischemia-Reperfusion Injury by Restricting Y-Box Binding Protein-1 Ubiquitination.Journal of the American Society of Nephrology : JASN · 2015Article
- The role of cold shock domain proteins in inflammatory diseases.Journal of molecular medicine (Berlin, Germany) · 2014Review
- The proteolytic YB-1 fragment interacts with DNA repair machinery and enhances survival during DNA damaging stress.Cell cycle (Georgetown, Tex.) · 2013Article
- Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities.Cell communication and signaling : CCS · 2013Article
- PDGFR-α inhibition preserves blood-brain barrier after intracerebral hemorrhage.Annals of neurology · 2011Article
- Y-box protein-1/p18 fragment identifies malignancies in patients with chronic liver disease.BMC cancer · 2011Article
- Article
- YB-1 is a Transcription/Translation Factor that Orchestrates the Oncogenome by Hardwiring Signal Transduction to Gene Expression.Translational oncogenomics · 2007Article
- Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species.Molecular and cellular biology · 2006Article
- Y-box factor YB1 controls p53 apoptotic function.Oncogene · 2005Article
- Proteasome-mediated cleavage of the Y-box-binding protein 1 is linked to DNA-damage stress response.The EMBO journal · 2005Article
- Identification of a novel Rev-interacting cellular protein.BMC cell biology · 2005Article
- Inhibition of protein synthesis by Y box-binding protein 1 blocks oncogenic cell transformation.Molecular and cellular biology · 2005Article
- Y box-binding protein 1 induces resistance to oncogenic transformation by the phosphatidylinositol 3-kinase pathway.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Functional interaction between JC virus late regulatory agnoprotein and cellular Y-box binding transcription factor, YB-1.Journal of virology · 2002Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have recently demonstrated that thrombin induces expression of the platelet-derived growth factor B-chain gene in endothelial cells (EC) through activation of the Y-box binding protein DNA-binding protein B (dbpB). We now present evidence that dbpB is activated by a novel mechanism: proteolytic cleavage leading to release from mRNA, nuclear translocation, and induction of thrombin-responsive genes. Cytosolic, full-length dbpB (50 kDa) was rapidly cleaved to a 30-kDa species upon thrombin stimulation of EC. This truncated, "active" dbpB exhibited nuclear localization and binding affinity for the thrombin response element sequence, which is distinct from the Y-box sequence. Oligo(dT) affinity chromatography revealed that cytosolic dbpB from control EC, but not active dbpB from thrombin-treated EC, was bound to mRNA. Latent dbpB immunoprecipitated from cytosolic extracts of control EC was activated by ribonuclease treatment. Furthermore, when EC cytosolic extracts were subjected to Nycodenz gradient centrifugation, latent dbpB fractionated with mRNA, whereas active dbpB fractionated with free proteins. The cytosolic retention domain of dbpB, which we localized to the region 247-267, was proteolytically cleaved during its activation. In contrast to full-length dbpB, truncated dbpB stimulated platelet-derived growth factor B-chain and tissue factor promoter activity by over 5-fold when transiently cotransfected with reporter constructs. These results suggest a novel mode of transcription factor activation in which an agonist causes release from mRNA of a latent transcription factor leading to its transport to the nucleus and its regulation of target gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.