ArticleMolecular and cellular biology2001
TFIIS enhances transcriptional elongation through an artificial arrest site in vivo.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Chromatin regulates alternative polyadenylation via the RNA polymerase II elongation rate.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Centromeric and pericentric transcription and transcripts: their intricate relationships, regulation, and functions.Chromosoma · 2023Review
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- Pause & go: from the discovery of RNA polymerase pausing to its functional implications.Current opinion in cell biology · 2017Review
- Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin.PLoS genetics · 2015Article
- Fail-safe transcription termination: Because one is never enough.RNA biology · 2015Review
- PHD and TFIIS-Like domains of the Bye1 transcription factor determine its multivalent genomic distribution.PloS one · 2014Article
- Basic mechanisms of RNA polymerase II activity and alteration of gene expression in Saccharomyces cerevisiae.Biochimica et biophysica acta · 2013Review
- The prefoldin complex regulates chromatin dynamics during transcription elongation.PLoS genetics · 2013Article
- Article
- Dissection of Pol II trigger loop function and Pol II activity-dependent control of start site selection in vivo.PLoS genetics · 2012Article
- Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin.Nature structural & molecular biology · 2011Article
- Using positional distribution to identify splicing elements and predict pre-mRNA processing defects in human genes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- An evolutionarily 'young' lysine residue in histone H3 attenuates transcriptional output in Saccharomyces cerevisiae.Genes & development · 2011Article
- Article
- The peptidyl prolyl isomerase Rrd1 regulates the elongation of RNA polymerase II during transcriptional stresses.PloS one · 2011Article
- The transcription factor Spn1 regulates gene expression via a highly conserved novel structural motif.Journal of molecular biology · 2010Article
- Article
- Stability, flexibility, and dynamic interactions of colliding RNA polymerase II elongation complexes.Molecular cell · 2009Article
- RNA polymerase active center: the molecular engine of transcription.Annual review of biochemistry · 2009Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptional elongation by RNA polymerase II has been well studied in vitro, but understanding of this process in vivo has been limited by the lack of a direct and specific assay. Here, we designed a specific assay for transcriptional elongation in vivo that involves an artificial arrest (ARTAR) site designed from a thermodynamic theory of DNA-dependent transcriptional arrest in vitro. Transcriptional analysis and chromatin immunoprecipitation experiments indicate that the ARTAR site can arrest Pol II in vivo at a position far from the promoter. TFIIS can counteract this arrest, thereby demonstrating that it possesses transcriptional antiarrest activity in vivo. Unexpectedly, the ARTAR site does not function under conditions of high transcriptional activation unless cells are exposed to conditions (6-azauracil or reduced temperature) that are presumed to affect elongation in vivo. Conversely, TFIIS affects gene expression under conditions of high, but not low, transcriptional activation. Our results provide physical evidence for the discontinuity of transcription elongation in vivo, and they suggest that the functional importance of transcriptional arrest sites and TFIIS is strongly influenced by the level of transcriptional activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.