ArticleJournal of virology2001
Nef enhances human immunodeficiency virus type 1 infectivity resulting from intervirion fusion: evidence supporting a role for Nef at the virion envelope.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- PF74 Reinforces the HIV-1 Capsid To Impair Reverse Transcription-Induced Uncoating.Journal of virology · 2018Article
- HIV: Antiviral action countered by Nef.Nature · 2015Article
- Article
- The activity of Nef on HIV-1 infectivity.Frontiers in microbiology · 2014Review
- Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells.Bio-protocol · 2013Article
- The frantic play of the concealed HIV envelope cytoplasmic tail.Retrovirology · 2013Review
- Comparative proteomic analysis of HIV-1 particles reveals a role for Ezrin and EHD4 in the Nef-dependent increase of virus infectivity.Journal of virology · 2013Article
- The host proteins transportin SR2/TNPO3 and cyclophilin A exert opposing effects on HIV-1 uncoating.Journal of virology · 2013Article
- A hunter virus that targets both infected cells and HIV free virions: implications for therapy.Theoretical biology & medical modelling · 2012Article
- HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation.PloS one · 2012Article
- Nef can enhance the infectivity of receptor-pseudotyped human immunodeficiency virus type 1 particles.Journal of virology · 2008Article
- Identification of a CCR5-expressing T cell subset that is resistant to R5-tropic HIV infection.PLoS pathogens · 2007Article
- Evidence for a functional link between uncoating of the human immunodeficiency virus type 1 core and nuclear import of the viral preintegration complex.Journal of virology · 2006Article
- A mutation in the human immunodeficiency virus type 1 Gag protein destabilizes the interaction of the envelope protein subunits gp120 and gp41.Journal of virology · 2006Article
- Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Detection of human immunodeficiency virus type 1 Nef and CD4 physical interaction in living human cells by using bioluminescence resonance energy transfer.Journal of virology · 2005Article
- Role of accessory proteins of HTLV-1 in viral replication, T cell activation, and cellular gene expression.Frontiers in bioscience : a journal and virtual library · 2004Review
- The membrane-proximal tyrosine-based sorting signal of human immunodeficiency virus type 1 gp41 is required for optimal viral infectivity.Journal of virology · 2004Article
- Small-molecule inhibition of human immunodeficiency virus type 1 replication by specific targeting of the final step of virion maturation.Journal of virology · 2004Article
- Nef does not affect the efficiency of human immunodeficiency virus type 1 fusion with target cells.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef stimulates viral infectivity by facilitating an early event in the HIV-1 life cycle. Although no structural or biochemical defects in Nef-defective HIV-1 particles have been demonstrated, the Nef protein is incorporated into HIV-1 particles. To localize the function of Nef within the virus particle, we developed a novel technology involving fusion of enveloped donor HIV-1 particles bearing core defects with envelope-defective target virions bearing HIV-1 receptors. Although neither virus alone was capable of infecting CD4(+) target cells, the incubation of donor and target virions prior to addition to target cells resulted in infection. This effect, termed "virion transcomplementation," required a functional Env protein on the donor virus and CD4 and an appropriate coreceptor on target virions. To provide evidence for intervirion fusion as the mechanism of complementation, experiments were performed using dual-enveloped HIV-1 particles bearing both HIV-1 and ecotropic murine leukemia virus (E-MLV) Env proteins as donor virions. Infection of CD4-negative target cells bearing E-MLV receptors was prevented by HIV-1 entry inhibitors when added before, but not after, incubation of donor and target virions prior to the addition to cells. When we used Nef(+) and Nef(-) donor and target virions, Nef enhanced infection when present in donor virions. In contrast, no effect of Nef was detected when present in the target virus. These results reveal a potential mechanism for enhancing HIV-1 diversity in vivo through the rescue of defective viral genomes and provide a novel genetic system for the functional analysis of virion-associated proteins in HIV-1 infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.