ArticleThe Journal of physiology2001
Role of myoplasmic phosphate in contractile function of skeletal muscle: studies on creatine kinase-deficient mice.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 99 citations in OpenAlex.
- Severity of arterial hypoxaemia affects the relative contributions of peripheral muscle fatigue to exercise performance in healthy humans.The Journal of physiology · 2007Trial
- Muscle cramps as disorders of impaired termination of contraction: An integrated neurophysiological framework.Physiological reports · 2026Review
- The influence of biological sex on the metabolic basis of skeletal muscle fatigue in vivo.The Journal of physiology · 2025Article
- Skeletal Muscle Fatigue in Rats Is More Consistently Related to Increased Inorganic Phosphate Concentration Than Acidosis.Acta physiologica (Oxford, England) · 2025Article
- The addition of blood flow restriction during resistance exercise does not increase prolonged low-frequency force depression.Experimental physiology · 2024Article
- Fatigue in CKD: Epidemiology, Pathophysiology, and Treatment.Clinical journal of the American Society of Nephrology : CJASN · 2021Review
- Maximal muscular power: lessons from sprint cycling.Sports medicine - open · 2021Review
- Quantification of creatine kinase reaction rate in mouse hindlimb using phosphorus-31 magnetic resonance spectroscopic fingerprinting.NMR in biomedicine · 2021Article
- Inverse Correlation Between Grip Strength and Serum Phosphorus: A Retrospective Observational Study in Japanese Elderly with Poorly Controlled Type 2 Diabetes.Geriatrics (Basel, Switzerland) · 2020Article
- Role of CaPflugers Archiv : European journal of physiology · 2018Article
- Exploring the Link between Serum Phosphate Levels and Low Muscle Strength, Dynapenia, and Sarcopenia.Scientific reports · 2018Article
- Molecular Basis for Exercise-Induced Fatigue: The Importance of Strictly Controlled Cellular CaCold Spring Harbor perspectives in medicine · 2018Review
- Energetics of muscle contraction: further trials.The journal of physiological sciences : JPS · 2017Review
- Faster and stronger manifestation of mitochondrial diseases in skeletal muscle than in heart related to cytosolic inorganic phosphate (Pi) accumulation.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Kinetic changes in tetanic Ca²⁺ transients in enzymatically dissociated muscle fibres under repetitive stimulation.The Journal of physiology · 2011Article
- Similar mitochondrial activation kinetics in wild-type and creatine kinase-deficient fast-twitch muscle indicate significant Pi control of respiration.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
- Does central fatigue exist under low-frequency stimulation of a low fatigue-resistant muscle?European journal of applied physiology · 2010Article
- Deficit in human muscle strength with cast immobilization: contribution of inorganic phosphate.European journal of applied physiology · 2006Article
- Increased resistance to fatigue in creatine kinase deficient muscle is not due to improved contractile economy.Pflugers Archiv : European journal of physiology · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Increased myoplasmic inorganic phosphate (P(i)) has been suggested to have an important role in skeletal muscle fatigue, especially in the early phase. In the present study we used intact fast-twitch muscle cells from mice completely deficient in creatine kinase (CK(-/-)) to test this suggestion. These CK(-/-) muscle cells provide a good model since they display a higher P(i) concentration in the unfatigued state and fatigue without significant increase of P(i). 2. Tetanic contractions (350 ms duration) were produced in intact single muscle fibres. The free myoplasmic [Ca(2+)] ([Ca(2+)](i)) was measured with the fluorescent indicator indo-1. The force-[Ca(2+)](i) relationship was constructed from tetani at different frequencies. 3. Compared with wild-type fibres, CK(-/-) fibres displayed lower force in 100 Hz tetani and at saturating [Ca(2+)](i) (i.e. 100 Hz stimulation during caffeine exposure), higher tetanic [Ca(2+)](i) during the first 100 ms of tetanic stimulation, reduced myofibrillar Ca(2+) sensitivity when measurements were performed 100-200 ms into tetani, and slowed force relaxation that was due to altered cross-bridge kinetics rather than delayed Ca(2+) removal from the myoplasm. 4. In wild-type fibres, a series of 10 tetani resulted in reduced tetanic force, slowed force relaxation, and increased amplitude of [Ca(2+)](i) tails after tetani. None of these changes were observed in CK(-/-) fibres. 5. Complementary experiments on isolated fast-twitch extensor digitorum longus muscles showed a reduction of tetanic force and relaxation speed in CK(-/-) muscles similar to those observed in single fibres. 6. In conclusion, increased P(i) concentration can explain changes observed in the early phase of skeletal muscle fatigue. Increased P(i) appears to be involved in both fatigue-induced changes of cross-bridge function and SR Ca(2+) handling.
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