ArticleThe EMBO journal2001
Transfer RNA(Ala) recognizes transfer-messenger RNA with specificity; a functional complex prior to entering the ribosome?
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Structures of tmRNA and SmpB as they transit through the ribosome.Nature communications · 2021Article
- Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment withProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Origins of tmRNA: the missing link in the birth of protein synthesis?Nucleic acids research · 2016Review
- Accommodation of tmRNA-SmpB into stalled ribosomes: a cryo-EM study.RNA (New York, N.Y.) · 2010Article
- Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Two-piece tmRNA in cyanobacteria and its structural analysis.Nucleic acids research · 2002Article
- SmpB functions in various steps of trans-translation.Nucleic acids research · 2002Article
- The tmRNA Website: invasion by an intron.Nucleic acids research · 2002Article
- Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging.Nucleic acids research · 2001Article
- Structure and function of the T-loop structural motif in noncoding RNAs.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors.
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Abstract
tmRNA (SsrA or 10Sa RNA) functions as both a transfer RNA and a messenger RNA, rescues stalled ribosomes and clears the cell of incomplete polypeptides. We report that native Escherichia coli tmRNA interacts specifically with native or synthetic E.coli tRNA alanine (tRNA(Ala)) in vitro, alanine being the first codon of the tmRNA internal open reading frame. Aminoacylatable RNA microhelices also bind tmRNA. Complex formation was monitored by gel retardation assays combined with structural probes. Nucleotides from the acceptor stem of tRNA(Ala) are essential for complex formation with tmRNA. tRNA(Ala) isoacceptors recognize tmRNA with different affinities, with an important contribution from tRNA(Ala) post-transcriptional modifications. The most abundant tRNA(Ala) isoacceptor in vivo binds tmRNA with the highest affinity. A complex between tRNA(Ala) and tmRNA might involve up to 140 tmRNA molecules out of 500 present per E.coli cell. Our data suggest that tmRNA interacts with the tRNA that decodes the resume codon prior to entering the ribosome. Biological implications of promoting specific complexes between tmRNA and aminoacylatable RNAs are discussed, with emphasis on primitive versions of the translation apparatus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.