ArticleThe EMBO journal2001
Determinants for hairpin formation in Tn10 transposition.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation.PloS one · 2023Article
- Biochemical Characterization of Kat1: a Domesticated hAT-Transposase that Induces DNA Hairpin Formation and MAT-Switching.Scientific reports · 2016Article
- The processing of repetitive extragenic palindromes: the structure of a repetitive extragenic palindrome bound to its associated nuclease.Nucleic acids research · 2012Article
- H-NS mediates the dissociation of a refractory protein-DNA complex during Tn10/IS10 transposition.Nucleic acids research · 2011Article
- Preventing broken Borrelia telomeres: ResT couples dual hairpin telomere formation with product release.The Journal of biological chemistry · 2010Article
- Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.Critical reviews in biochemistry and molecular biology · 2010Review
- Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif.Nucleic acids research · 2008Article
- Requirements for DNA hairpin formation by RAG1/2.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Base-flipping dynamics in a DNA hairpin processing reaction.Nucleic acids research · 2007Article
- Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates.Nucleic acids research · 2006Article
- The global regulator H-NS acts directly on the transpososome to promote Tn10 transposition.Genes & development · 2005Article
- Mixing active-site components: a recipe for the unique enzymatic activity of a telomere resolvase.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Excision of Sleeping Beauty transposons: parameters and applications to gene therapy.The journal of gene medicine · 2004Article
- Patterns of sequence conservation at termini of long terminal repeat (LTR) retrotransposons and DNA transposons in the human genome: lessons from phage Mu.Nucleic acids research · 2003Article
- IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration.The EMBO journal · 2002Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tn10 transposition involves the formation of a hairpin intermediate at the transposon termini. Here we show that hairpin formation exhibits more stringent DNA sequence requirements at the terminal two base pairs than either transpososome assembly or first strand nicking. We also observe a significant DNA distortion at the terminal base pairs upon transpososome assembly by chemical nuclease footprinting. Interestingly, mutations at these positions do not necessarily inhibit the formation of the distortion. However, it remains a possibility that the inhibitory effect of these mutations is due to a defect in protein-DNA interactions subsequent to this deformation. Terminal base pair mutations also inhibited strand transfer, providing evidence that transposase interactions with the terminal residues on both 'transferred' and 'non-transferred' strands are important for hairpin formation. We also demonstrate that mutation of a highly conserved tyrosine residue that is a component of the YREK motif, Y285, results in a phenotype comparable to that of the terminal base pair mutations. In contrast, a mutation at another conserved position, W265, is shown to relax the specificity of the hairpin formation reaction.
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