ArticleThe EMBO journal2001
Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Mysterious and fascinating: DNA polymerase ɩ remains enigmatic 20 years after its discovery.DNA repair · 2020Review
- Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue HChemical research in toxicology · 2020Article
- Mouse DNA polymerase ι lacking the forty-two amino acids encoded by exon-2 is catalytically inactive in vitro.DNA repair · 2017Article
- Review
- The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.Oncogene · 2014Article
- Replication across regioisomeric ethylated thymidine lesions by purified DNA polymerases.Chemical research in toxicology · 2013Article
- Article
- A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases.Nucleic acids research · 2009Article
- Y-family DNA polymerases in mammalian cells.Cellular and molecular life sciences : CMLS · 2009Review
- Molecular breeding of polymerases for amplification of ancient DNA.Nature biotechnology · 2007Article
- Translesion synthesis past tamoxifen-derived DNA adducts by human DNA polymerases eta and kappa.Biochemistry · 2006Article
- Hepatitis C virus induces a mutator phenotype: enhanced mutations of immunoglobulin and protooncogenes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis.The EMBO journal · 2003Article
- 129-derived strains of mice are deficient in DNA polymerase iota and have normal immunoglobulin hypermutation.The Journal of experimental medicine · 2003Article
- Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase iota.Nucleic acids research · 2002Article
- Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Human DNA polymerase mu (Pol mu) exhibits an unusual replication slippage ability at AAF lesion.Nucleic acids research · 2002Article
- Somatic immunoglobulin hypermutation.Current opinion in immunology · 2002Review
- Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
A hallmark of human DNA polymerase iota (poliota) is the asymmetric fidelity of replication at template A and T when the enzyme extends primers annealed to a single-stranded template. Here, we report on the efficiency and accuracy of poliota-dependent replication at a nick, a gap, the very end of a template and from a mispaired primer. Poliota cannot initiate synthesis on a nicked DNA substrate, but fills short gaps efficiently. Surprisingly, poliota's ability to blunt-end a 1 bp recessed terminus is dependent upon the template nucleotide encountered and is highly erroneous. At template G, both C and T are inserted with roughly equal efficiency, whilst at template C, C and A are misinserted 8- and 3-fold more often than the correct base, G. Using substrates containing mispaired primer termini, we show that poliota can extend all 12 mispairs, but with differing efficiencies. Poliota can also extend a tandem mispair, especially when it is located within a short gap. The enzymatic properties of poliota appear consistent with that of a somatic hypermutase and suggest that poliota may be one of the low-fidelity DNA polymerases hypothesized to participate in the hypermutation of immunoglobulin variable genes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.